General Info
Host: | Rabbit |
Applications: | WB/IHC/IF |
Reactivity: | Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Rabbit polyclonal antibody anti-BCAR1 (495-612) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
Purification: | Affinity purification |
Dilution Range: | WB 1:100-1:500IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Information
Gene Symbol: | BCAR1 |
Gene ID: | 9564 |
Uniprot ID: | BCAR1_HUMAN |
Immunogen Region: | 495-612 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 495-612 of human BCAR1 (NP_055382.2). |
Immunogen Sequence: | EPQEPLVQDLQAAVAAVQSA VHELLEFARSAVGNAAHTSD RALHAKLSRQLQKMEDVHQT LVAHGQALDAGRGGSGATLE DLDRLVACSRAVPEDAKQLA SFLHGNASLLFRRTKATA |
Description
Tissue Specificity | Expressed in B-cells (at protein level). Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. |
Post Translational Modifications | PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif.phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix. Dephosphorylated by PTPN14 at Tyr-128. Phosphorylated by SRC kinase in a EDN1- and PTK2B-mediated manner.phosphorylation strengthens its interaction with BCAR3 as part of the PTK2B/BCAR1/BCAR3/RAP1 signaling pathway. |
Function | Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration and cell branching. Involved in the BCAR3-mediated inhibition of TGFB signaling. |
Protein Name | Breast Cancer Anti-Estrogen Resistance Protein 1Crk-Associated SubstrateCas Scaffolding Protein Family Member 1P130cas |
Database Links | Reactome: R-HSA-186763Reactome: R-HSA-372708Reactome: R-HSA-4420097Reactome: R-HSA-8849471 |
Cellular Localisation | Cell JunctionFocal AdhesionCytoplasmCell ProjectionAxonUnphosphorylated Form Localizes In The CytoplasmLocalizes To Focal Adhesion Sites Following Integrin Engagement |
Alternative Antibody Names | Anti-Breast Cancer Anti-Estrogen Resistance Protein 1 antibodyAnti-Crk-Associated Substrate antibodyAnti-Cas Scaffolding Protein Family Member 1 antibodyAnti-P130cas antibodyAnti-BCAR1 antibodyAnti-CAS antibodyAnti-CASS1 antibodyAnti-CRKAS antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance