Human Obesity Array C1
Katalog-Nummer AAH-ADI-1-8
Size : 8SampleKit
Marke : RayBiotech
Contact local distributor :
Telefonnummer : +1 850 650 7790
Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Human |
| Number of Targets Detected | 62 |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Membrane |
| Method Of Detection | Chemiluminescence |
| Design Principle | Sandwich-based |
| Research Area | Cardiovascular Disease, Obesity |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
4-1BB (TNFRSF9/CD137), ACE-2, Adiponectin (ACRP30), Adipsin (Complement Factor D), AgRP, Angiopoietin-1, Angiopoietin-2, ANGPTL4, CRP (C-Reactive Protein), ENA-78 (CXCL5), Fas (TNFRSF6/Apo-1), FGF-6, Growth Hormone, HCC-4 (CCL16), IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGF-1, IGF-1 R, IL-1 R4 (ST2), IL-1 R1, IL-10, IL-11, IL-12 p70, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-6, IL-6 R, IL-8 (CXCL8), Insulin, IP-10 (CXCL10), Leptin R, Leptin, LIF, Lymphotactin (XCL1), MCP-1 (CCL2), MCP-3 (MARC/CCL7), M-CSF, MIF, MIP-1 beta (CCL4), MSP alpha/beta, Osteoprotegerin (TNFRSF11B), Oncostatin M, PAI-1, PARC (CCL18), PDGF-AA, PDGF-AB, PDGF-BB, RANTES (CCL5), Resistin, SAA (Serum Amyloid A), SDF-1 alpha (CXCL12 alpha), TNF RII (TNFRSF1B), TNF RI (TNFRSF1A), TECK (CCL25), TGF beta 1, TIMP-1, TIMP-2, TNF alpha, VEGF-A, XEDAR
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Obesity Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Citations
Catalioto, Rose-Marie, et al. "Characterization of a novel proinflammatory effect mediated by BK and the kinin B2 receptor in human preadipocytes." Biochemical pharmacology86.4 (2013): 508-520.
Species:
Human
Sample Type:
Conditioned Media (pre-adipocytes obesity model)
Li S., Ding S., Mersmann H., et al. A nutritional nonalcoholic steatohepatitis minipig model. J Nutr Biochem. 2016 Feb;28:51-60. doi: 10.1016/j.jnutbio.2015.09.029
Species:
Porcine
Sample Type:
Plasma (Minipig dietary model)
Park H., Park H., Pak H., et al. miR-34a inhibits differentiation of human adipose tissue-derived stem cells by regulating cell cycle and senescence induction. Differentiation. 2015 Nov-Dec;90(4-5):91-100. doi: 10.1016/j.diff.2015.10.010.
Species:
Human
Sample Type:
Conditioned Media (Adipose tissue lysate stem cells)
Bauer D., Mazzio E., Soliman K., Taka E., Oriaku E., et al. Diallyl Disulfide Inhibits TNF?-induced CCL2 Release by MDA-MB-231 Cells. ANTICANCER RESEARCH 34: 2763-2770 (2014)
Species:
Human
Sample Type:
Conditioned Media (MDA-MB-231 Cells)
Al?Rakan, Maha A., et al. "Breast stromal fibroblasts from histologically normal surgical margins are pro?carcinogenic." The Journal of pathology 231.4 (2013): 457-465.
Species:
Human
Sample Type:
Cell Lysate (SFCMs from CAF, TCF and NBF cells)