Human Obesity Array C1

Katalog-Nummer AAH-ADI-1-8

Size : 8SampleKit

Marke : RayBiotech

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Telefonnummer : +1 850 650 7790
Human Obesity Array C1

Human Obesity Array C1

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1  Review (include details such as sample type used, experimental conditions, etc.)
Cytokine array for detection of 62 human adipokines. Suitable for all liquid sample types.
Only %1 left

Product Description


Size 2 Sample Kit, 4 Sample Kit, 8 Sample Kit
Species Human
Number of Targets Detected 62
Quantitative/Semi-Quantitative Semi-Quantitative
Compatible Sample Types Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Solid Support Membrane
Method Of Detection Chemiluminescence
Design Principle Sandwich-based
Research Area Cardiovascular Disease, Obesity

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

4-1BB (TNFRSF9/CD137), ACE-2, Adiponectin (ACRP30), Adipsin (Complement Factor D), AgRP, Angiopoietin-1, Angiopoietin-2, ANGPTL4, CRP (C-Reactive Protein), ENA-78 (CXCL5), Fas (TNFRSF6/Apo-1), FGF-6, Growth Hormone, HCC-4 (CCL16), IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGF-1, IGF-1 R, IL-1 R4 (ST2), IL-1 R1, IL-10, IL-11, IL-12 p70, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-6, IL-6 R, IL-8 (CXCL8), Insulin, IP-10 (CXCL10), Leptin R, Leptin, LIF, Lymphotactin (XCL1), MCP-1 (CCL2), MCP-3 (MARC/CCL7), M-CSF, MIF, MIP-1 beta (CCL4), MSP alpha/beta, Osteoprotegerin (TNFRSF11B), Oncostatin M, PAI-1, PARC (CCL18), PDGF-AA, PDGF-AB, PDGF-BB, RANTES (CCL5), Resistin, SAA (Serum Amyloid A), SDF-1 alpha (CXCL12 alpha), TNF RII (TNFRSF1B), TNF RI (TNFRSF1A), TECK (CCL25), TGF beta 1, TIMP-1, TIMP-2, TNF alpha, VEGF-A, XEDAR

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Obesity Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis


For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.


Catalioto, Rose-Marie, et al. "Characterization of a novel proinflammatory effect mediated by BK and the kinin B2 receptor in human preadipocytes." Biochemical pharmacology86.4 (2013): 508-520.
Sample Type:
Conditioned Media (pre-adipocytes obesity model)
Li S., Ding S., Mersmann H., et al. A nutritional nonalcoholic steatohepatitis minipig model. J Nutr Biochem. 2016 Feb;28:51-60. doi: 10.1016/j.jnutbio.2015.09.029
Sample Type:
Plasma (Minipig dietary model)
Park H., Park H., Pak H., et al. miR-34a inhibits differentiation of human adipose tissue-derived stem cells by regulating cell cycle and senescence induction. Differentiation. 2015 Nov-Dec;90(4-5):91-100. doi: 10.1016/j.diff.2015.10.010.
Sample Type:
Conditioned Media (Adipose tissue lysate stem cells)
Bauer D., Mazzio E., Soliman K., Taka E., Oriaku E., et al. Diallyl Disulfide Inhibits TNF?-induced CCL2 Release by MDA-MB-231 Cells. ANTICANCER RESEARCH 34: 2763-2770 (2014)
Sample Type:
Conditioned Media (MDA-MB-231 Cells)
Al?Rakan, Maha A., et al. "Breast stromal fibroblasts from histologically normal surgical margins are pro?carcinogenic." The Journal of pathology 231.4 (2013): 457-465.
Sample Type:
Cell Lysate (SFCMs from CAF, TCF and NBF cells)

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