NADP/NADPH BioAssay™ Kit (Nicotinamide Adenine Dinucleotide Phosphate)

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+/NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue.

Cell or tissue extracts

Direct Assays: NADP+/NADPH concentrations and ratios in cell or tissue extracts.

Detection limit 0.1um, linearity up to 10um

This assay is highly specific for NADP+/NADPH and is not interfered by NAD+/NADH.

This NADP+/NADPH BioAssay™ kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565nm, is proportionate to the NADP+/NADPH concentration in the sample. This assay is a convenient method to measure NADP, NADPH and their ratio.

Assay Buffer, 1x10ml
Glucose (1M), 1x1.5ml
MTT Solution, 1x1.5ml
Enzyme Mix, 1x120ul
NADP Standard, 1mM, 1x0.5ml
NADP/NADPH Extraction Buffers, 12ml each

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.