Dusp1 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN304862BN

Dusp1 - mouse gene knockout kit via CRISPR, HDR mediated

Functional Cassette: GFP-puro Luciferase-Puro RFP-BSD mBFP-Neo



HDR-mediated knockout kit validation

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Specifications

Product Data
Format 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
Donor DNA mBFP-Neo
Symbol Dusp1
Locus ID 19252
Components

KN304862G1, Dusp1 gRNA vector 1 in pCas-Guide CRISPR vector

KN304862G2, Dusp1 gRNA vector 2 in pCas-Guide CRISPR vector

KN304862BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_013642
UniProt ID P28563
Synonyms 3CH134; erp; mkp-1; MKP1; Ptpn16; U19515
Summary Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.[UniProtKB/Swiss-Prot Function]

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