Mouse IL-1RA/IL1RN ELISA Kit

Specifications

Product Data
Assay Type	Sandwich ELISA kit of Quantitative Detection for Mouse IL-1RA/IL1RN
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	62.5pg/ml-4000pg/ml
Sample Type	Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of mouse IL-1RA
Sensitivity	<10pg/ml
Reactivities	Mouse
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- mouse IL-1RAantibody 1 Lyophilized recombinant mouse IL-1RA standard 10ng/tubex2 Biotinylated anti- mouse IL-1RA antibody 130ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) Sample diluent buffer 30 ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml
Background	The interleukin-1 receptor antagonist(IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The human IL1RN gene was localized to the long arm of chromosome 2 at band 2q14.2 by fluorescence in situ hybridization. IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha(IL1A) and interleukin 1, beta(IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses.
Gene Symbol	Il1rn
Gene ID	16181