CCR3 ELISA Kit (Mouse)
Katalog-Nummer OKEH05256
Size : 96Wells
Marke : Aviva Systems Biology
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| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Mouse, Mus musculus | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Ccr3 ELISA Kit (Mouse) (OKEH05256) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ccr3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ccr3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ccr3 captured in the well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | <= 6.8% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision. Mean Intra-Assay Precision - <4.5%, n = 20 Mean Inter-Assay Precision - <6.4%, n = 20 | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.18 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Mouse Probable C-C chemokine receptor type 3 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | Ccr3 |
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| Gene Full Name | chemokine (C-C motif) receptor 3 |
| Alias Symbols | CC chemokine receptor 3;C-C CKR-3;CC-CKR;CC-CKR3;CC-CKR-3;CCR-3;chemokine (C-C) receptor 1,-like 2;chemokine (C-C) receptor 3;CKR;CKR3;Cmkbr;Cmkbr1l2;Cmkbr3;macrophage inflammatory protein 1-alpha receptor-like 2;MIP-1 alpha RL2;MIP-1 alphaRL2;probable C-C chemokine receptor type 3. |
| NCBI Gene Id | 12771 |
| Protein Name | P51678 |
| Description of Target | Probable C-C chemokine receptor type 3 |
| Uniprot ID | https://www.uniprot.org/uniprot/P51678 |
| Protein Accession # | NP_034044.3 |
| Nucleotide Accession # | NM_009914.4 |
| Protein Size (# AA) | 359 |