SensoLyte® Plus 520 MMP-2 Assay Kit Fluorimetric and Enhanced Selectivity - 1 kit

Katalog-Nummer AS-72224

Size : 1kit

Marke : AnaSpec

Contact local distributor :

Telefonnummer : +1 850 650 7790

Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc
endopeptidases capable of digesting extracellular matrix components. MMP-2 (72-kDa gelatinase-A/type IV collagenase) is responsible for degradation of collagen, fibronectin, laminin, and elastin. It has been shown that MMP-2 plays a key role in angiogenesis, tumor cell invasion, and metastasis. MMP-2 is also considered a therapeutic target for cancer.
The SensoLyte® Plus 520 MMP-2 Assay Kit is designed specifically for detecting MMP-2 activity in biological samples, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate, which may contain multiple MMPs. Members of the MMP family have poor substrate
sequence specificity, thus using peptide substrate alone to differentiate the activity of a particular MMP from other MMPs is inadequate. A monoclonal anti-human-MMP-2 antibody is therefore employed to pull down MMP-2 from the mixture. MMP-2 activity is then quantified by a 5-FAM/QXL™ 520 fluorescence resonance energy transfer (FRET) peptide (Scheme 1). The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assay can detect as low as 3 ng/mL active MMP-2 enzyme. The assay has no cross-reactivity with human MMP-1, 3, 7, 8, 9, 10, 12, 13, 15, 16 and has minimal cross-reactivity with MMP-14).


Kits components
  • Component A: Microplate coated with monoclonal anti human MMP-2 antibody: 12 x 8 black strips Component B: MMP-2 standard, recombinant human pro-MMP-2: 10 µg/mL, 15 µL Component C: Assay buffer: 50 mL Component D: 10X Wash buffer: 50 mL Component E: APMA, 4-aminophenylmercuric acetate (Caution: Contain organic mercury. Dispose it according to your local regulations): 100 mM, 150 µL Component F: MMP-2 substrate 5-FAM/QXL™520 FRET peptide Ex/Em=490 nm/520 nm upon cleavage: 50 µL Component G: Stop Solution: 10 mL Component H: Adhesive cover strip: 3 sheets
UniProt number
  • P08253
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store Component B at -80°C. Store all other components at -20°C. Components D, G, H can be stored at RT for convenience.
  • Protease activity
Biomarker Target
  • Matrix Metalloproteinase (MMP)
  • MMP-2
Detection Method
  • Fluorescent
Detection Limit
  • 3 ng/ml
Research Area
  • Adhesion & Extracellular Matrix
  • Cancer & Apoptosis
Sub-category Research Area
  • Oncogenesis
  • Protein Assay Kits
  • Tissue Remodeling/MMPs
  • Research use
  • CHO (mammalian cells)

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