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AP Chromogen for IHC - Fast Red

AP Chromogen for IHC - Fast Red


Fast Red chromogenic substrate is an alkaline phosphatase substrate used for immunohistochemical (IHC) revelation. When reacted with alkaline phosphatase, Fast Red produces an insoluble red precipitate at the reaction site on tissues and cells.

AP hydrolyzes the naphthol phosphate esters to phenolics and phosphates. The phenols couple to colorless diazonium salts to produce an insoluble colored azo dye.

The appearance of the staining can be followed by observation under the microscope.

The precipitate formed by Fast Red in the presence of alkaline phosphatase is soluble in alcohol.

Fast Red can be used to make multiple labeling with other chromogens.


Name
Fast Red
Applications
IHC, ICC, ISH, WB, ELISA
Detection enzyme
Alkaline phosphatase
Reaction type
Precipitating Reaction
Precipitate color
Red
Microscopy
Brightfield, Spectral Imaging
Mounting
Aqueous
Other properties
Contrast with Tissue Pigments, Multiple Labeling

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