Human PLA2R1 (Secretory phospholipase A2 receptor) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	PLA2R1
Gene ID:	22925
Uniprot ID:	PLA2R_HUMAN
Specificity:	This kit recognizes Human PLA2R1 in samples. No significant cross-reactivity or interference between Human PLA2R1 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Expressed in podocytes (at protein level). Present in lung macrophage (at protein level). Highly expressed in kidney. Also expressed in pancreas, amnion, choriodecidua and placenta. Isoform 2 is expressed at much lower level.
Post Translational Modifications	The secretory phospholipase A2 receptor form may be produced by the action of metalloproteinases. It contains all extracellular domains and only lacks transmembrane and cytosolic regions. It is however unclear whether this form is produced by proteolytic cleavage as suggested by some experiments, or by alternative splicing, as in the case of isoform 2 that shares all characteristics of secretory phospholipase A2 receptor form.
Function	Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phospholipase sPLA2-IB/PLA2G1B but not sPLA2-IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in pro-inflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B. In podocytes, binding of sPLA2-IB/PLA2G1B can regulate podocyte survival and glomerular homeostasis.
Protein Name	Secretory Phospholipase A2 Receptor Pla2-R Pla2r 180 Kda Secretory Phospholipase A2 Receptor C-Type Lectin Domain Family 13 Member C M-Type Receptor Cleaved Into - Soluble Secretory Phospholipase A2 Receptor Soluble Pla2-R Soluble Pla2r
Database Links	Reactome: R-HSA-1482788 Reactome: R-HSA-1482801 Reactome: R-HSA-1482839 Reactome: R-HSA-1482922 Reactome: R-HSA-1482925 Reactome: R-HSA-1483166
Cellular Localisation	Cell Membrane Single-Pass Type I Membrane Protein Soluble Secretory Phospholipase A2 Receptor: Secreted Isoform 2: Secreted
Alternative CLIA Names	Secretory Phospholipase A2 Receptor CLIA kit Pla2-R CLIA kit Pla2r CLIA kit 180 Kda Secretory Phospholipase A2 Receptor CLIA kit C-Type Lectin Domain Family 13 Member C CLIA kit M-Type Receptor Cleaved Into - Soluble Secretory Phospholipase A2 Receptor CLIA kit Soluble Pla2-R CLIA kit Soluble Pla2r CLIA kit PLA2R1 CLIA kit CLEC13C CLIA kit
Specificity	This kit recognizes Human PLA2R1 in samples. No significant cross-reactivity or interference between Human PLA2R1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	94-108	100
EDTA plasma(n=8)	92-106	98
Cell culture media(n=8)	102-115	108

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	92.17	304.56	900.12	99.51	278.42	875.37
Standard deviation	9.72	30.61	69.49	12.72	21.69	74.41
CV (%)	10.55	10.05	7.72	12.78	7.79	8.50

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance