Low-Cost Basics in Molecular Biology

NeoPrep mini

 NB-60-0001

• Neo Biotech's miniprep kits offer a fast and efficient way to extract pure plasmid DNA from Escherichia coli.
• The process includes alkaline lysis of bacterial cells and DNA binding to silica under high salt conditions.
• The resulting DNA is ideal for PCR, automated fluorescent sequencing, and various enzymatic applications.

NeoGPure

NB-60-0002

• The Neo Biotech Gelpure kit purifies DNA fragments from agarose gels and PCR products (50 bp to 20 kb) with a recovery rate of 60-90% (depending on fragment size).
• It uses a silica-gel membrane that selectively adsorbs DNA fragments(<20 μg).
• The kit's binding buffer includes a pH indicator for assessing optimal DNA binding pH without affecting the purification process.

Neoset dNTPs

NB-60-0004

• Ready-to-use molecular grade dNTP solutions for DNA polymerization.
• Contains dATP, dGTP, dCTP, and dTTP at a concentration of 100 mM.
• Supplied in sodium salt solution at pH 8.5 (at 22 °C).
• Ideal for DNA labeling and sequencing applications.     

NeoProof DNA polymerase

NB-60-0006

• NeoProof DNA polymerase: a recombinant enzyme with high fidelity and excellent PCR performance.
• Includes 3´→5´ exonuclease proofreading for improved accuracy.
• Efficiently amplifies longer PCR products (≤10 kb) and enables site-directed mutagenesis.
• Recommended for routine cloning.
• Error rate comparable to Pfu and Kod DNA polymerases, but significantly lower than Taq DNA polymerases.

Neogreen qPCR Master Mix (2x)

NB-60-0003-01

• qPCR Green Master Mix (2x) is a Defined reaction mixture for real-time PCR.
• It is highly efficient and optimized for optimal performance.
• The mix includes a hot-start enzyme control mechanism, enhancing detection sensitivity.
• Incorporates the latest advancements in PCR enhancer technology, such as buffer chemistry and robustly engineered enzymes.
• Engineered to deliver precise and dependable results for accurate analysis.

NeoTaqII DNA polymerase

NB-60-0005

• The new generation of Taq-derived DNA polymerases optimized for standard PCR applications.
• Engineered to produce high DNA yields in shorter PCR running times (15-30 s/kb extension).
• Lacks 3´→5´ exonuclease activity and supports reliable amplification of a wide range of DNA templates up to 6 kb.
• Suitable for PCR under minimal optimization conditions.

NeoSpin

• NeoSpin centrifugal filters from Neo Biotech:
• Efficient and rapid sample preparation
• Polypropylene housing with a polyethersulfone (PES) membrane
• High flow rates and high protein retention
• Heat-sealed membrane eliminates adhesives and is downstream extractable
• Compatible with pH 1 to 9
• Enables maximum protein recovery without introducing pipetting errors
• Low binding membrane and polypropylene housing for >90% sample recovery

5KD NB-57-0006

10KD NB-57-0007 

30KD NB-57-0009

50KD  NB-57-0008

100KD NB-57-0010

5KD NB-57-0001-2

10KD NB-57-0002-2   

30KD NB-57-0003-2

50KD NB-57-0004-2

100KD NB-57-0005-2

NeoTrap

• Designed for simple, one-step, and rapid purification of immunoglobulins
• Traps proteins through covalent binding to prevent sample loss
• Allows capture of broad source species and IgG subclasses
• Enables column reusing
• Compatible with standard liquid chromatography instruments (including ÄKTA™ FPLC's), peristaltic pumps, and syringes.

NeoTrap Protein G

FPLC Columns

NeoTrap Protein A

FPLC Column

NeoTrap Ni-NTA

FPLC Column

NeoTrap Co-IDA

FPLC Column

NeoPRO Western ECL Substrate

• The NeoPRO Western Substrate is a luminol-based enhanced chemiluminescent substrate.
• It is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP)–conjugated secondary antibodies.
• Enables mid-femtogram to high-femtogram detection of antigen.
• Offers excellent sensitivity and long signal duration.
• Allows for digital and film-based imaging without signal loss.
• No optimization is required for use.
• Provides a high degree of sensitivity and enhanced chemiluminescence duration.
• Optimized for use with PVDF and nitrocellulose membranes.
• Compatible with Western Blot Markers.
• Optimized for film and CCD-based imaging.

NeoPRO4

NB-12-8001

NeoPRO Mini Vertical Protein Electrophoresis System:

• Used for pre-casting and hand-casting gels
• Allows for up to 4 gel pieces to be run simultaneously
• Compatible with 1-D and 2-D electrophoresis applications
• Gel-casting frame and spacer glass plate simplify hand-casting and prevent leakage                                                                                                                                                      

NeoBlot Mini

NB-12-8002

• Part of the Neo Biotech mini electrophoresis system
• Accommodates 2 blot clips
• Used for protein and RNA sample blotting
• Four transfer plates are available
• High-current fast transfer and low-current overnight transfer options
• Built-in freeze block for efficient heat absorption                            
• Easy-to-use lock mechanism in the gel clamp                                                                                                                                                                                                                                                  

Quick Coomassie Stain

• Rapid staining - Protein bands appear after a few minutes - Fully visualization of the staining after 1 hour
• Compassionate - 20 ng protein resolution
• Accurate protein quantification - Low background - Linear staining range
• Re-useable - Up to 3 times
• Storage - up to 6 months at RT or up to 1 year at 4ºC                                                                                                                                                                                    

NeoPRO 10 Prestained Protein Ladder

NB-59-0002

• The NeoPRO 10 Prestained Protein Ladder is a combination of 10 pre-stained proteins with molecular weights ranging from 6.5 to 270 kDa.
• The 10 recombinant proteins in the ladder are covalently coupled with a blue chromophore.
• The ladder includes 2 orange bands at 30 kDa and 270 kDa, as well as a green band at 52 kDa, which function as reference bands.
• It is designed to monitor the size and separation of proteins during SDS-polyacrylamide gel electrophoresis.
• The NeoPRO 10 Prestained Protein Ladder helps estimate protein size and validate the efficiency of Western transfer on PVDF, nylon, or nitrocellulose membranes.