Free Secondary Antibody with Every Primary Antibody Bought

Primary antibodies specifically recognize and bind target antigens such as cell surface markers, signaling molecules, and modified proteins, enabling detection and isolation using techniques like western blot, ELISA, immunoprecipitation, and flow cytometry. They are classified as polyclonal, recognizing multiple epitopes, or monoclonal, which bind a single epitope. Primary antibodies can be directly labeled for detection or paired with secondary antibodies for indirect detection. Secondary antibodies, typically polyclonal, target primary antibodies from specific host species and isotypes. Produced by immunizing one species with antibodies from another, they are labeled with reporter molecules (enzymes, fluorescent dyes) to generate measurable signals in applications such as western blot, IHC, ICC, ELISA, and flow cytometry. Indirect detection using secondary antibodies offers signal amplification, as multiple secondary antibodies can bind a single primary antibody, enhancing sensitivity.

Take advantage of this limited-time promotion: receive a secondary antibody from SouthernBiotch with any primary antibody purchase.

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