Even small RNAs can be sequenced !!!

Even small RNAs can be sequenced !!!

New generation of reverse transcriptasefor RNA-Seq

SPECIAL OFFER 40% off on TGIRT® III products*

*Offer n°20190218-TGIRT valid until 31/03/2019

TGIRT® III enzyme is a new generation of reverse transcriptase with several advantages :

  • Simultaneously reverse transcribing and adding an RNA-Seq adapter to RNAs of all sizes and structures
  • Less biased manner than other methods
  • Make it possible to obtain full-length reads of tRNAs and other structured non-coding RNAs


2 different variations of the method

  • One for RNA-Seq of small RNAs : PAGE-purified cDNAs of selected sizes are circularized with CircLigaseII
  • The other for RNA-Seq of total RNAs of all size classes



Available in 2 formats : enzyme alone and kit

Description
Size
Product code
10 µl
TGIRT10
  
50 µl
TGIRT50
  
5x50 µl
TGIRT5x50
  
10x50 µl
TGIRT10x50
  
25 rxns
KTGIRT-25

**TGIRT Kit contents (10 reactions or 25 reactions):
  • TGIRT®-III enzyme, 1 x 10 µl (KTGIRT-10) or 3 x 10 µl (KTGIRT-25)
  • 10X Primer mix, 50 µl (PM-110)
  • 10 x DTT, 50 µl (D-121)
  • 5 x Reaction Buffer, 100 µl (RX-120)