Protease assay kits

Protease assay kits

For drug target screening and discovery

We are pleased to spotlight our wide portfolio of iD SensoLyte® Protease Activity Assay kits for the study of proteases having roles in specific diseases. Further, our Custom iD Optimization services caters to custom assay development with high quality and minimal lead time.



Catalog & custom protease activity assays - Features & benefits

  • Ready-to-use mix and read homogeneous format ideal for HTS.
  • All essential components for performing assays in 96-well plate formats.
  • Detailed protocols and references included, ensuring value and reliability.
  • Proprietary substrates (FRET pairs) in many of our protease kits.
  • Custom assay design and development offering a full spectrum of dyes and quenchers covering all detection channels.

Protease assay kits


SensoLyte® Catalog Protease Kits & Related Services

  • Targeted Catalog Assay Kits:  iD SensoLyte® catalog Protease Assay kits feature proteases that have important roles in
- Neuroscience (Alzheimer's, Parkinson's MS),
- Cancer,
- Epigenetics,
- Cell adhesion & ECM (MMPs),
- Cardiovascular/Metabolic and 
- Viral pathogenesis.

  • Bulk FRET or fluorogenic substrates:  For developing your own HTS assays, we conveniently offer substrates separately from our assay kits.

  • iD Optimization and Screening:  includes optimizing and scale up of any SensoLyte® assay kit, and small molecules screening with choice of assay kit.

SensoLyte® Catalog Protease Assay Kits

Neuroscience

Type
Product
Product code
Cathepsins
AS-72164;72165

Cell adhesion & ECM

Type
Product
Product code
Cathepsins
AS-72164;72165

Cancer & Apoptosis

Type
Product
Product code

Cardiovascular & metabolic related

Type
Product
Product code
Factor Xa                                        
AS-72207
AS-72208
Renin                                                                                                                                                             
AS-72040
AS-72039
AS-72140
AS-72161
DPPIV                                        
AS-72196
AS-72197

Epigenetics

Type
Product
Product code

Viral & bacterial proteases

Type
Product
Product code

Role of Proteases

Proteases are enzymes that catalyze the hydrolysis of peptide and isopeptide bonds that link amino acids within proteins, in the process referred to as proteolysis. Proteases have evolved as multifunctional enzymes adapting and diversifying to various biological roles resultant of changes in their expression patterns, substrate specificities and structure-function relationships.Their roles include,

  • Activating cytokines and receptors for cell signaling.
  • Cell death mediators and Antigen processors: 
- Activation of Bid in apoptosis by Caspases-8, Calpains & Cathepsins B, L S, H, D, acting via the loops or interdomain linker regions. 
- Processing antigens via the lysosomal system for presentation by MHC class II molecules, such as cathepsins and deubiquitinating enzymes.

  • Ecdomomain processors by exposing cryptic sites, such as beta-secretase during amyloid precursor protein processing or Shedding various transmembrane proteins such as metalloproteases and cysteine proteases.
  • ECM modulators by using exosites as effective substrate binding,such as thrombin, ADAMTS-4 and most MMPs.
  • Targets for viral inhibitors to arrest viral proteases processing viral polyproteins in causing pathogenicity.


Figure 2.Schematic representation of diverse roles of Proteases.