Plasma Kallikrein Activity Assay Kit

Plasma Kallikrein Activity Assay Kit

Sensitive detection of plasma Kallikrein & inhibitors screening

SensoLyte® Rh110 Plasma Kallikrein Activity Assay Kit is designed to detect human plasma kallikrein in biological fluids.



It also includes a plasma kallikrein inhibitor and protocol which are suited for potential pharmacotherapy studies and compound screening.

Benefits :  
  • Fast & convenient
  • Sensitivity : 1 ng/ml
  • Fluorometric detection

Features :
  • Detection of plasma kallikrein
  • Compound screening for plasma kallikrein inhibitors

Plasma kallikrein detection

As an important vascular protease/biomarker, Plasma kallikrein represents the kallikrein-kinin system responsible for liberating bradykinin, a potent vasoactive pro-inflammatory peptide. It's detection in plasma lends an important indication of the cardiovascular state involving alternate and classical coagulation pathways.

Kallikrein-1
Fig 1. Kallikrein activity present in plasma samples was detected by SensoLyte® Rh110 Plasma Kallikrein Activity Assay Kit. Plasma samples were diluted by 1X Assay buffer. The fluorescence signal was measured after 1 hour incubation of samples with substrate.

Kallikrin-2
Fig 2. The specificity of SensoLyte® Rh110 Plasma Kallikrein Acitivty Assay Kit was tested with prekallikrein depleted human plasma. Lack of prekallikrein in the depleted plasma resulted in no conversion of prekallikrein to kallikrein.

Plasma Kallikrein inhibitors screening

Imbalance between plasma kallikrein and its naturally occurring plasma inhibitors is associated with disease states including hereditary angioedema (HAE), systemic lupus, inflammatory bowel disease and rheumatoid arthritis.
Our kit also provides an effective and convenient way to screen for plasma kallikrein inhibitors in your compound(s) of interest.

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Fig 3. Inhibition of Kallikrein activity by PKSI-527 measured with SensoLyte® Rh110 Plasma Kallikrein Activity Assay Kit. Fluorescence signal was detected at Ex/Em=490/520nm (SpectraMax M5e).

About Kallikrein :

Plasma Kallikrein is implicated in several processes including blood coagulation, cardiovascular and cerebrovascular physiological and pathological phenomena. It is actively involved in the initiation of classical and activation of the alternate complement pathways.

Plasma kallikrein is an important vascular protease that represents the kallikrein-kinin proteolytic system responsible for liberating bradykinin, a vasoactive pro-inflammatory peptide.

Imbalance between plasma kallikrein and its naturally occurring plasma inhibitors is associated with disease states including hereditary angioedema (HAE), systemic lupus, inflammatory bowel disease and rheumatoid arthritis.

Kallikrein-4

Plasma Kallikrein is considered as a vascular biomarker:

1. Activated plasma kallikrein initiates surface mediated activation of coagulation, fibrinolysis and kinin generation.
2. Plasma kallikrein converts prorenin to renin, thus modulating activity of renin angiotensin aldosterone system (RAAS) at various levels.
3. Plasma kallikrein accelerates activation of FXII towards fibrinolytic mechanism.