Immunohistochemistry / Immunofluorescence

Immunohistochemistry / Immunofluorescence


Immunohistochemistry (IHC) General protocol

Immunohistochemistry protocol - Avidin/Biotin Method (ABC)
The principle of ABC detection kits is based on the affinity of avidin (or streptavidin) for biotin. Indeed, abbreviation ABC stands for Avidin / Biotin Complex. In general, the ABC detection kits are composed of: - A biotinylated secondary antibody - An Avidin / Enzyme Complex The kits may or may not contain the blocking and dilution buffers and the substrate depending on the formats.