
Immunohistochemistry / Immunofluorescence

The principle of ABC detection kits is based on the affinity of avidin (or streptavidin) for biotin. Indeed, abbreviation ABC stands for Avidin / Biotin Complex.
In general, the ABC detection kits are composed of: - A biotinylated secondary antibody - An Avidin / Enzyme Complex
The kits may or may not contain the blocking and dilution buffers and the substrate depending on the formats.
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