Human LDHA (L-lactate dehydrogenase A chain) CLIA Kit

Katalog-Nummer NB-22-40513-1

Size : 96T

Marke : Neo Biotech

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Telefonnummer : +1 850 650 7790

  • Human LDHA (L-lactate dehydrogenase A chain) CLIA Kit

General Info

Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This human LDHA kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence

Information

Gene Symbol: LDHA
Gene ID: 3939
Uniprot ID: LDHA_HUMAN
Specificity: This kit recognizes Human LDHA in samples. No significant cross-reactivity or interference between Human LDHA and analogues was observed.
Sample Type: Serum, plasma and other biological fluids

Description

Tissue Specificity Predominantly expressed in anaerobic tissues such as skeletal muscle and liver.
Post Translational Modifications ISGylated.
Function Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
Protein Name L-Lactate Dehydrogenase A Chain
Ldh-A
Cell Proliferation-Inducing Gene 19 Protein
Ldh Muscle Subunit
Ldh-M
Renal Carcinoma Antigen Ny-Ren-59
Database Links Reactome: R-HSA-70268
Cellular Localisation Cytoplasm
Alternative CLIA Names L-Lactate Dehydrogenase A Chain CLIA kit
Ldh-A CLIA kit
Cell Proliferation-Inducing Gene 19 Protein CLIA kit
Ldh Muscle Subunit CLIA kit
Ldh-M CLIA kit
Renal Carcinoma Antigen Ny-Ren-59 CLIA kit
LDHA CLIA kit
PIG19 CLIA kit
Specificity This kit recognizes Human LDHA in samples. No significant cross-reactivity or interference between Human LDHA and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-102 96
EDTA plasma(n=8) 97-111 105
Cell culture media(n=8) 92-106 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.46 1.30 4.76 0.46 1.24 5.18
Standard deviation 0.05 0.09 0.50 0.06 0.10 0.54
CV (%) 10.87 6.92 10.50 13.04 8.06 10.42
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance