Mouse genotyping

Mouse genotyping

Accurate identification of genetically modified animals is critical for effective research and the reduction of animal usage in experimental studies. In general, genotype determination is achieved through DNA analysis, primarily from young rodent tissues. Polymerase chain reaction (PCR) analysis is the most common method used for this purpose and requires a small quantity of DNA. Tissue samples such as tail biopsies, ear perforations, hairs, blood, fecal or oral specimens can be used for PCR analysis.

The isolation of DNA from transgenic mouse samples is a challenging process that has been addressed by numerous studies with varying degrees of success. Silica membrane column purification methods can effectively eliminate contaminants and inhibitors but are costly and may have low yield. Cheaper alternatives such as sodium hydroxide (NaOH) and proteinase K (PK) can save time, but contamination can lead to inconsistent results.

To address this issue, we propose PCR genotyping kits that include the necessary reagents for DNA extraction from various samples (tail, ear, hair, blood, etc.) and the amplification of specific sequences.


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