HRP Chromogen for IHC - DAB

HRP Chromogen for IHC - DAB



DAB (3,3'-Diaminobenzidine) is the most widely used chromogen for use with HRP. DAB substrate forms a brown colored deposit when it reacts with peroxidase in the presence of hemoglobin.
The use of DAB is simple, simply incubate the slides for 5 minutes in a humid chamber at room temperature. The appearance of the staining can be followed by observation under the microscope.
When staining is complete, it is necessary to rinse the slides with water for 1-2 minutes to remove excess DAB. The precipitate from DAB is insoluble in alcohol and other organic solvents. Samples can be dehydrated and slides mounted permanently (O-Mount).

Name
DAB (3,3'-Diaminobenzidine)
Applications
IHC, ICC, ISH, WB, ELISA
Detection enzyme
HRP
Reaction type
Precipitating Reaction
Precipitate color
Brown
Microscopy
Brightfield, Darkfield, Electron, Spectral Imaging
Mounting
Aqueous (Hardening), Non-Aqueous (Permanent)
Other properties
Heat Resistant, Multiple Labeling

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RUOCE / IVD
  • Unconjugated 855
  • HRP 112
  • Biotin 41
  • FITC 40
  • APC 35
  • PE 35
  • AP 30
  • MaxLight 405 28
  • MaxLight 490 28
  • MaxLight 550 28
  • MaxLight 650 28
  • MaxLight 750 28
  • Tue 8
  • Cy3 5
  • 13C 3
  • APC/Cy7 2
  • AbFluor™ 488 1
  • AbFluor™ 594 1
  • Alexa Fluor 647 1
  • Alexa Fluor 750 1
  • human 707
  • mouse 208
  • rat 199
  • e. coli 49
  • cow 40
  • dog 38
  • pig 38
  • rabbit 29
  • cat 26
  • horse 20
  • camel 16
  • hamster 16
  • monkey 16
  • sheep 16
  • guinea pig 7
  • plant 5
  • chicken 4
  • duck 4
  • fish 4
  • goose 4
  • pigeon 4
  • monosan dab high contrast 2
  • 1 1
  • monosan plus (hrp) broad spectrum (dab)kit 1
  • human 344
  • mouse 329
  • rabbit 289
  • virus 97
  • 48
  • yeast 34
  • goat 33
  • e. coli 20
  • hamster 14
  • rat 13
  • horse 12
  • sheep 12
  • dog 10
  • chicken 8
  • cow 8
  • monkey 8
  • pig 8
  • bird 5
  • guinea pig 3
  • cat 1
  • donkey 1
  • Protein/peptide 970
  • Primary antibody 716
  • Biochemicals 531
  • Inhibitor/Antagonist/Agonist 304
  • ELISA/ELISPOT 262
  • CRISPR/Cas9 177
  • Buffers and reagents 122
  • RNAi 104
  • kit 98
  • cDNA Clone 69
  • Other products 28
  • Destination vector 24
  • Inhibitor / Antagonist / Agonist 24
  • Secondary antibody 19
  • Custom services 14
  • Cell line 12
  • Probe 6
  • pcr products 3
  • Discontinued 2
  • Lysate 2
  • Paraffin tissue section 1
  • Primer 1
  • Service 1
  • ELISA 511
  • WB 453
  • IHC 294
  • FLISA 82
  • ICC 68
  • IP 66
  • FC 56
  • IF 32
  • PCR 4
  • Dot blot 3
  • Polyclonal 328
  • 10i46 22
  • 13H261 22
  • 1F8 22
  • 1G1 22
  • 1G5 22
  • 2G6 22
  • 3H1166 22
  • 4G5 22
  • HLy9.1.25 8
  • Monoclonal 7
  • 10.1F8 4
  • 12H6.D3 4
  • 13.1G11 4
  • 13.2G8 4
  • 13.6D9 4
  • 13.7E2 4
  • 13.7H4 4
  • 13.8E5 4
  • 13B4.E10 4
  • 14.2H9 4
  • 14F10.H9 4
  • 1A6 4
  • 26.7C4 4
  • 2E4 4
  • 3G8 4
  • 4A8 4
  • 4B9 4
  • 5A11 4
  • 5A2 4
  • 6.1H3.D9.C6 4
  • 6.1H8.F9.G6 4
  • 6.2D2.E1.D11 4
  • 6.7A2.A4.D3 4
  • 6.8A2.B8.A11 4
  • 6.8E7.A7.A1 4
  • 8C7 4
  • 8G1 4
  • 8H12 4
  • 8H6 4
  • 9.21C1.G1.F1.A5 4
  • 9.22E1.F5.H10 4
  • 9.24E10.F3.H12 4
  • CRF Anti-Polyvalent HRP Polymer (DAB) Lab Pack 4
  • IgG.2A5 4
  • IgG.2E9 4
  • 3
  • 12K274 2
  • 2213 2
  • 2M2 2
  • 3F9-2C2 2
  • 8C10 2
  • B2M-01 2
  • B2M-02 2
  • DAB (Post) Enhancing Solution 2
  • ADAB-8 1
  • CRF Anti-Polyvalent HRP Polymer (DAB) Stain Kit 1
  • DAB Away Kit 1
  • DAB Differentiating Solution 1
  • DAB Enhancer Solution 1
  • DAB-6 1
  • EconoTek HRP Anti-Polyvalent (DAB) 1
  • SensiTek HRP Anti-Polyvalent (DAB) Staining System 1


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