HRP Chromogen for IHC - DAB
DAB (3,3'-Diaminobenzidine) is the most widely used chromogen for use with HRP. DAB substrate forms a brown colored deposit when it reacts with peroxidase in the presence of hemoglobin.
The use of DAB is simple, simply incubate the slides for 5 minutes in a humid chamber at room temperature. The appearance of the staining can be followed by observation under the microscope.
When staining is complete, it is necessary to rinse the slides with water for 1-2 minutes to remove excess DAB. The precipitate from DAB is insoluble in alcohol and other organic solvents. Samples can be dehydrated and slides mounted permanently (O-Mount).
Name
DAB (3,3'-Diaminobenzidine)
Applications
IHC, ICC, ISH, WB, ELISA
Detection enzyme
HRP
Reaction type
Precipitating Reaction
Precipitate color
Brown
Microscopy
Brightfield, Darkfield, Electron, Spectral Imaging
Mounting
Aqueous (Hardening), Non-Aqueous (Permanent)
Other properties
Heat Resistant, Multiple Labeling
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RUOCE / IVD
- Unconjugated 833
- HRP 98
- Biotin 66
- FITC 62
- PE 51
- APC 48
- MaxLight 490 39
- MaxLight 550 39
- MaxLight 650 39
- AP 30
- MaxLight 405 28
- MaxLight 750 28
- Cy3 7
- APC/Cy7 2
- APC-CY5.5 1
- APC-Cy7 1
- Alexa Fluor 350 1
- Alexa Fluor 405 1
- Alexa Fluor 488 1
- Alexa Fluor 555 1
- Alexa Fluor 594 1
- Alexa Fluor 647 1
- Alexa Fluor 680 1
- Alexa Fluor 750 1
- Cy5 1
- Cy5.5 1
- Cy7 1
- PE-Cy3 1
- PE-Cy5 1
- PE-Cy5.5 1
- PE-Cy7 1
- PerCP-Cy5.5 1
- PerCP-Cy7 1
- PerCp 1
- RBITC 1
- human 976
- mouse 281
- rat 260
- cow 50
- e. coli 49
- dog 48
- pig 48
- cat 27
- rabbit 25
- horse 23
- monkey 19
- sheep 18
- camel 16
- hamster 16
- guinea pig 7
- chicken 6
- plant 5
- donkey 4
- duck 4
- fish 4
- goose 4
- pigeon 4
- monosan dab high contrast 2
- xenopus 2
- zebrafish 2
- 1 1
- monosan plus (hrp) broad spectrum (dab)kit 1
- Primary antibody 1048
- Protein/peptide 967
- Biochemicals 743
- ELISA/ELISPOT 315
- Inhibitor/Antagonist/Agonist 289
- CRISPR/Cas9 269
- RNAi 184
- Buffers and reagents 74
- cDNA Clone 51
- Destination vector 50
- kit 44
- Other products 22
- Inhibitor / Antagonist / Agonist 18
- Cell line 12
- Probe 6
- Lysate 2
- Paraffin tissue section 1
- Primer 1
- mouse 499
- rabbit 441
- human 402
- virus 97
- 48
- rat 46
- e. coli 38
- yeast 34
- goat 33
- horse 12
- dog 10
- hamster 10
- sheep 10
- cow 8
- monkey 8
- pig 8
- bird 5
- chicken 5
- cat 1
- donkey 1
- guinea pig 1
- WB 637
- ELISA 625
- IHC 354
- FLISA 109
- IP 93
- ICC 79
- FC 76
- IF 56
- Enzyme-linked immunosorbent assay for Antigen Detection. 10
- 0.078 ng/mL 3
- 0.1 ng/mL 2
- 0.116 ng/ml 1
- PCR 1
- Polyclonal 499
- 2G6 32
- 4G5 32
- 1F8 31
- 1G1 31
- 1G5 31
- 10i46 26
- 13H261 22
- 3H1166 22
- M-A261 12
- Monoclonal 12
- HLy9.1.25 6
- 1A10A10 5
- 10.1F8 4
- 12H6.D3 4
- 13.1G11 4
- 13.2G8 4
- 13.6D9 4
- 13.7E2 4
- 13.7H4 4
- 13.8E5 4
- 13B4.E10 4
- 14.2H9 4
- 14F10.H9 4
- 1A6 4
- 26.7C4 4
- 2E4 4
- 3G8 4
- 4A8 4
- 4B9 4
- 5A11 4
- 5A2 4
- 6.1H3.D9.C6 4
- 6.1H8.F9.G6 4
- 6.2D2.E1.D11 4
- 6.7A2.A4.D3 4
- 6.8A2.B8.A11 4
- 6.8E7.A7.A1 4
- 8C7 4
- 8G1 4
- 8H12 4
- 8H6 4
- 9.21C1.G1.F1.A5 4
- 9.22E1.F5.H10 4
- 9.24E10.F3.H12 4
- IgG.2A5 4
- IgG.2E9 4
- 6D23 3
- SAA1697 3
- 12K274 2
- 1626CT702.263.21 2
- 17D17 2
- 17L18 2
- 202.36 2
- 2213 2
- 2M2 2
- 3F9-2C2 2
- 5B20 2
- 7H111 2
- 8C10 2
- B2M-01 2
- B2M-02 2
- [HLy9.1.25] 2
- 11C257 1
- 12K99 1
- 134-2C2 1
- 13B822 1
- 5K78 1
- 5K78 (134-2C2) 1
- 6C1 1
- 6C5 1
- 9H177 1
- ADAB-8 1
- DAB-6 1
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