HRP Chromogen for IHC - DAB
DAB (3,3'-Diaminobenzidine) is the most widely used chromogen for use with HRP. DAB substrate forms a brown colored deposit when it reacts with peroxidase in the presence of hemoglobin.
The use of DAB is simple, simply incubate the slides for 5 minutes in a humid chamber at room temperature. The appearance of the staining can be followed by observation under the microscope.
When staining is complete, it is necessary to rinse the slides with water for 1-2 minutes to remove excess DAB. The precipitate from DAB is insoluble in alcohol and other organic solvents. Samples can be dehydrated and slides mounted permanently (O-Mount).
Name
DAB (3,3'-Diaminobenzidine)
Applications
IHC, ICC, ISH, WB, ELISA
Detection enzyme
HRP
Reaction type
Precipitating Reaction
Precipitate color
Brown
Microscopy
Brightfield, Darkfield, Electron, Spectral Imaging
Mounting
Aqueous (Hardening), Non-Aqueous (Permanent)
Other properties
Heat Resistant, Multiple Labeling
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- Unconjugated 673
- HRP 46
- Biotin 10
- APC 5
- Alexa Fluor 350 5
- Alexa Fluor 488 5
- Alexa Fluor 555 5
- Alexa Fluor 750 5
- Cy3 5
- Cy5 5
- Cy5.5 5
- Cy7 5
- FITC 5
- PE 5
- human 354
- e. coli 70
- cow 40
- camel 16
- dog 16
- hamster 16
- horse 16
- monkey 16
- pig 16
- sheep 16
- plant 5
- cat 4
- chicken 4
- donkey 4
- duck 4
- fish 4
- goose 4
- pigeon 4
- yeast 3
- monosan dab high contrast 2
- virus 2
- 1 1
- monosan plus (hrp) broad spectrum (dab)kit 1
- human 239
- yeast 206
- e. coli 134
- 36
- horse 12
- virus 11
- dog 10
- hamster 10
- sheep 10
- cow 8
- monkey 8
- pig 8
- bird 5
- cat 1
- chicken 1
- donkey 1
- guinea pig 1
- Biochemicals 1258
- Protein/peptide 1086
- ELISA/ELISPOT 350
- Inhibitor/Antagonist/Agonist 264
- Primary antibody 250
- Buffers and reagents 101
- kit 98
- cDNA Clone 19
- Secondary antibody 12
- RNAi 10
- Other products 8
- Probe 6
- Lipids 2
- Lysate 2
- CRISPR/Cas9 1
- Paraffin tissue section 1
- Primer 1
- ELISA 358
- IHC 80
- WB 32
- IP 8
- IF 4
- 0.078 ng/mL 3
- Dot blot 3
- 0.1 ng/mL 2
- 0.116 ng/ml 1
- PCR 1
- Polyclonal 22
- 10.1F8 4
- 12H6.D3 4
- 13.1G11 4
- 13.2G8 4
- 13.6D9 4
- 13.7E2 4
- 13.7H4 4
- 13.8E5 4
- 13B4.E10 4
- 14.2H9 4
- 14F10.H9 4
- 1A6 4
- 26.7C4 4
- 2E4 4
- 3G8 4
- 4A8 4
- 4B9 4
- 5A11 4
- 5A2 4
- 6.1H3.D9.C6 4
- 6.1H8.F9.G6 4
- 6.2D2.E1.D11 4
- 6.7A2.A4.D3 4
- 6.8A2.B8.A11 4
- 6.8E7.A7.A1 4
- 8C7 4
- 8G1 4
- 8H12 4
- 8H6 4
- 9.21C1.G1.F1.A5 4
- 9.22E1.F5.H10 4
- 9.24E10.F3.H12 4
- IgG.2A5 4
- IgG.2E9 4
- 3
- Monoclonal 2
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