HRP Chromogen for IHC - DAB

HRP Chromogen for IHC - DAB



DAB (3,3'-Diaminobenzidine) is the most widely used chromogen for use with HRP. DAB substrate forms a brown colored deposit when it reacts with peroxidase in the presence of hemoglobin.
The use of DAB is simple, simply incubate the slides for 5 minutes in a humid chamber at room temperature. The appearance of the staining can be followed by observation under the microscope.
When staining is complete, it is necessary to rinse the slides with water for 1-2 minutes to remove excess DAB. The precipitate from DAB is insoluble in alcohol and other organic solvents. Samples can be dehydrated and slides mounted permanently (O-Mount).

Name
DAB (3,3'-Diaminobenzidine)
Applications
IHC, ICC, ISH, WB, ELISA
Detection enzyme
HRP
Reaction type
Precipitating Reaction
Precipitate color
Brown
Microscopy
Brightfield, Darkfield, Electron, Spectral Imaging
Mounting
Aqueous (Hardening), Non-Aqueous (Permanent)
Other properties
Heat Resistant, Multiple Labeling

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RUOCE / IVD
  • Unconjugated 843
  • HRP 127
  • Biotin 66
  • FITC 62
  • PE 51
  • APC 48
  • MaxLight 490 39
  • MaxLight 550 39
  • MaxLight 650 39
  • AP 30
  • MaxLight 405 28
  • MaxLight 750 28
  • Cy3 7
  • APC/Cy7 2
  • APC-CY5.5 1
  • APC-Cy7 1
  • Alexa Fluor 350 1
  • Alexa Fluor 405 1
  • Alexa Fluor 488 1
  • Alexa Fluor 555 1
  • Alexa Fluor 594 1
  • Alexa Fluor 647 1
  • Alexa Fluor 680 1
  • Alexa Fluor 750 1
  • Alexa Fluor® 350 1
  • Alexa Fluor® 488 1
  • Alexa Fluor® 555 1
  • Alexa Fluor® 594 1
  • Alexa Fluor® 647 1
  • Alexa Fluor® 680 1
  • Alexa Fluor® 750 1
  • Cy5 1
  • Cy5.5 1
  • Cy7 1
  • PE-Cy3 1
  • PE-Cy5 1
  • PE-Cy5.5 1
  • PE-Cy7 1
  • PerCP-Cy5.5 1
  • PerCP-Cy7 1
  • PerCp 1
  • RBITC 1
  • human 986
  • mouse 294
  • rat 277
  • cow 57
  • dog 55
  • pig 55
  • e. coli 49
  • horse 30
  • cat 27
  • rabbit 25
  • monkey 19
  • sheep 18
  • camel 16
  • hamster 16
  • guinea pig 14
  • chicken 6
  • plant 5
  • donkey 4
  • duck 4
  • fish 4
  • goose 4
  • pigeon 4
  • monosan dab high contrast 2
  • xenopus 2
  • zebrafish 2
  • 1 1
  • monosan plus (hrp) broad spectrum (dab)kit 1
  • Primary antibody 1064
  • Protein/peptide 967
  • Biochemicals 529
  • ELISA/ELISPOT 311
  • Inhibitor/Antagonist/Agonist 290
  • CRISPR/Cas9 269
  • RNAi 184
  • Buffers and reagents 74
  • kit 73
  • cDNA Clone 51
  • Destination vector 45
  • Other products 22
  • Inhibitor / Antagonist / Agonist 18
  • Cell line 12
  • Probe 6
  • Lysate 2
  • Paraffin tissue section 1
  • Primer 1
  • mouse 507
  • rabbit 462
  • human 399
  • virus 97
  • 48
  • rat 46
  • e. coli 38
  • goat 37
  • yeast 34
  • hamster 14
  • horse 12
  • sheep 12
  • dog 10
  • cow 8
  • monkey 8
  • pig 8
  • chicken 7
  • bird 5
  • guinea pig 3
  • cat 1
  • donkey 1
  • WB 652
  • ELISA 625
  • IHC 382
  • FLISA 109
  • IP 93
  • ICC 78
  • FC 76
  • IF 59
  • Enzyme-linked immunosorbent assay for Antigen Detection. 10
  • 0.078 ng/mL 3
  • 0.1 ng/mL 2
  • 0.116 ng/ml 1
  • PCR 1
  • Polyclonal 516
  • 2G6 32
  • 4G5 32
  • 1F8 31
  • 1G1 31
  • 1G5 31
  • 10i46 26
  • 13H261 22
  • 3H1166 22
  • M-A261 12
  • Monoclonal 11
  • HLy9.1.25 6
  • 1A10A10 5
  • 10.1F8 4
  • 12H6.D3 4
  • 13.1G11 4
  • 13.2G8 4
  • 13.6D9 4
  • 13.7E2 4
  • 13.7H4 4
  • 13.8E5 4
  • 13B4.E10 4
  • 14.2H9 4
  • 14F10.H9 4
  • 1A6 4
  • 26.7C4 4
  • 2E4 4
  • 3G8 4
  • 4A8 4
  • 4B9 4
  • 5A11 4
  • 5A2 4
  • 6.1H3.D9.C6 4
  • 6.1H8.F9.G6 4
  • 6.2D2.E1.D11 4
  • 6.7A2.A4.D3 4
  • 6.8A2.B8.A11 4
  • 6.8E7.A7.A1 4
  • 8C7 4
  • 8G1 4
  • 8H12 4
  • 8H6 4
  • 9.21C1.G1.F1.A5 4
  • 9.22E1.F5.H10 4
  • 9.24E10.F3.H12 4
  • IgG.2A5 4
  • IgG.2E9 4
  • 6D23 3
  • SAA1697 3
  • 12K274 2
  • 1626CT702.263.21 2
  • 17D17 2
  • 17L18 2
  • 202.36 2
  • 2213 2
  • 2M2 2
  • 3F9-2C2 2
  • 5B20 2
  • 7H111 2
  • 8C10 2
  • B2M-01 2
  • B2M-02 2
  • [HLy9.1.25] 2
  • 11C257 1
  • 12K99 1
  • 134-2C2 1
  • 13B822 1
  • 5K78 1
  • 5K78 (134-2C2) 1
  • 6C1 1
  • 6C5 1
  • 9H177 1
  • ADAB-8 1
  • DAB-6 1
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Katalog-Nummer
Beschreibung
Cond.
Preis zzgl. MwSt.
NB-23-00149
 100ml 
NB-23-00145-1
 1mg 
NB-23-00145-2
 500mL