HRP Chromogen - DAB-Ni

DAB (3,3'-Diaminobenzidine) is one of the most used substrates for peroxidase. Our DAB-Ni substrate is a DAB system enriched in nickel chloride with a higher sensitivity and contrast than conventional DAB. DAB-Ni forms a black deposit when it reacts with peroxidase.

The DAB-Ni substrate requires a 5 minute incubation in a humid chamber at room temperature. The appearance of the staining can be followed by observation under the microscope.

When good staining is achieved, it is necessary to rinse the slides with water for 1-2 minutes to remove excess DAB-Ni.

Like DAB, DAB-Ni is insoluble in alcohol and other organic solvents. Samples can be counter-stained, dehydrated and permanently mounted (O-Mount).

Name

DAB-Ni (3,3'-Diaminobenzidine + nickel chloride)

Applications

IHC, ICC, ISH, WB, ELISA

Detection enzyme

HRP

Reaction type

Precipitating Reaction

Precipitate color

Black

Microscopy

Brightfield, Darkfield, Electron, Spectral Imaging

Mounting

Aqueous (Hardening), Non-Aqueous (Permanent)

Other properties

Heat Resistant, Multiple Labeling

HRP Chromogen - DAB-Ni

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