HotStart High fidelity DNA polymerases

HotStart High fidelity DNA polymerases

Hot start DNA polymerase is a modified form of DNA polymerase which avoids a non-specific amplification of DNA by inactivation of the taq polymerase at low temperatures. The polymerase chain reaction (PCR) is a technology in molecular biology used to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. In Hot start PCR, specific antibodies are used to block the Taq-polymerase at lower temperature. An initial step at 95℃ is required for denaturing the antibodies linked to the active center of the enzyme. The anti-Taq antibodies reduce the Taq polymerase activity below 72℃, the optimal temperature at which the enzyme extends the primers. When the specific antibodies detach from Taq-polymerase, the amplification proceeds with greater specificity.

Different types of high-fidelity DNA polymerases are distinguished, including Pfu polymerase, a naturally faithful DNA polymerase due to its 3'-5 'exonuclease activity as well as modified DNA polymerases to obtain higher fidelity capacities.
The modifications made to the DNA polymerases to give a higher fidelity are different: genetic modification, chemical modification, mixing of several enzymes ...

NamePhanta HS Super-fidelity DNA polymerase
Product codeP502
TypeModified Pfu polymerase
Speed4 kb/min
Amplicon size< 2 kb
Error rate4.1x10-6 erreurs / nt
3'-5' exonuclease activityYes

Ergebnis Ihrer Suche : 3 Produkt(e) gefunden

Grenzen Sie Ihre Suche ein :

  • polymerase 2
  • Polymerase 2
  • pcr products 1
  • PCR 2

Preis zzgl. MwSt.