HotStart High fidelity DNA polymerases
Hot start DNA polymerase is a modified form of DNA polymerase which avoids a non-specific amplification of DNA by inactivation of the taq polymerase at low temperatures. The polymerase chain reaction (PCR) is a technology in molecular biology used to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. In Hot start PCR, specific antibodies are used to block the Taq-polymerase at lower temperature. An initial step at 95℃ is required for denaturing the antibodies linked to the active center of the enzyme. The anti-Taq antibodies reduce the Taq polymerase activity below 72℃, the optimal temperature at which the enzyme extends the primers. When the specific antibodies detach from Taq-polymerase, the amplification proceeds with greater specificity.
Different types of high-fidelity DNA polymerases are distinguished, including Pfu polymerase, a naturally faithful DNA polymerase due to its 3'-5 'exonuclease activity as well as modified DNA polymerases to obtain higher fidelity capacities.
The modifications made to the DNA polymerases to give a higher fidelity are different: genetic modification, chemical modification, mixing of several enzymes ...
|Name||Phanta HS Super-fidelity DNA polymerase|
|Type||Modified Pfu polymerase|
|Amplicon size||< 2 kb|
|Error rate||4.1x10-6 erreurs / nt|
|3'-5' exonuclease activity||Yes|
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- polymerase 2
- Polymerase 2
- pcr products 1
- PCR 2
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