F(ab) fragmented secondary antibodies

F(ab) fragmented secondary antibodies

Fab and F(ab')2 fragments are two types of fragmented secondary antibodies that are commonly used in various applications. Here are some key points about F(ab) fragments:

  • F(ab) fragments are monovalent antibody fragments that contain only one antigen-binding Fab portion and lack the Fc region. They are generated by papain digestion of whole IgG antibodies to remove the Fc region and the entire hinge region.
  • F(ab) fragments have a molecular weight of about 50 kDa, which is smaller than whole IgG antibodies and F(ab')2 fragments. This allows for better penetration into tissues and reduces nonspecific binding to Fc receptors on live cells or to Protein A/G.
  • F(ab) fragments are useful for blocking endogenous immunoglobulins on cells, tissues, or other surfaces, and for blocking the exposed immunoglobulins in multiple labeling experiments using primary antibodies from the same species. They can also be used to Fab-label primary antibodies prior to incubation with the experimental sample.
  • F(ab) fragments are monovalent and have lower avidity than F(ab')2 fragments, which may affect their sensitivity and specificity in certain applications.

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  • Unconjugated 236
  • FITC 82
  • Biotin 65
  • HRP 56
  • TRITC 34
  • AP 16
  • AMCA 10
  • Cy3 10
  • Cy5 10
  • Cy5.5 10
  • Cy7 10
  • Peroxidase 10
  • Red 10
  • PE 7
  • APC 6
  • AbFluor™ 488 6
  • AbFluor™ 594 6
  • Alexa Fluor 350 6
  • Alexa Fluor 405 6
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  • Alexa Fluor 647 6
  • Alexa Fluor 660 6
  • Alexa Fluor 680 6
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  • PerCp 4
  • human 285
  • mouse 68
  • rat 45
  • rabbit 38
  • goat 33
  • guinea pig 25
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  • hamster 20
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  • all species 10
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  • Secondary Antibody 4
  • goat 273
  • rabbit 139
  • mouse 126
  • human 35
  • donkey 32
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  • chicken 9
  • monkey 6
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  • IHC 397
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  • Polyclonal 439
  • Monoclonal 95
  • polyclonal 76
  • NI 614 (HP 614) 12
  • NI 6014 (HP 6014) 6
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Katalog-Nummer
Beschreibung
Cond.
Preis zzgl. MwSt.
NB-47-00228-1MG
 1mg 
800-101-098
 1mg