F(ab')2 fragment secondary antibodies

F(ab')2 fragment secondary antibodies

Secondary antibodies can be classified based on their specificity, which can be broad or narrow, and their fragment type, which can be whole, F(ab), or F(ab')2. 

The principle of F(ab')2 is to generate divalent antibody fragments that retain the antigen-binding Fab portions of whole IgG antibodies while removing most of the Fc region.

F(ab')2 fragments are generated by pepsin digestion of whole IgG antibodies to cleave the hinge region and leave intact some of the Fc region. The resulting fragments have two antigen-binding Fab portions linked together by disulfide bonds, making them divalent with a molecular weight of about 110 kDa. The use of F(ab')2 fragments has several advantages over whole IgG antibodies, including better penetration into tissues, reduced nonspecific binding to Fc receptors on live cells or to Protein A/G, and the ability to precipitate antigens. However, F(ab')2 fragments are not recommended for blocking since they have two binding sites that are available to capture the primary antibody introduced subsequently. The preparation of F(ab')2 fragments involves pepsin digestion of whole IgG antibodies, followed by purification using techniques such as FPLC with DEAE- 

 

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RUOCE / IVD
  • Unconjugated 1532
  • FITC 637
  • HRP 506
  • Biotin 441
  • AP 348
  • PE 171
  • Rhodamine 122
  • Red 115
  • TRITC 110
  • Texas Red® 81
  • RPE 71
  • Peroxidase 68
  • Colloidal Gold 60
  • LE/AF 23
  • APC 21
  • AbFluor™ 488 21
  • Alexa Fluor 555 20
  • Phosphatase 19
  • Alexa Fluor 647 18
  • Cy5 16
  • AMCA 15
  • SPRD 14
  • R-PE 13
  • AbFluor™ 594 12
  • Alexa Fluor 488 12
  • Cy3 12
  • R-Phycoerythrin 8
  • azide free 8
  • Phycoerythrin 7
  • Alexa Fluor 350 6
  • Alexa Fluor 405 6
  • Alexa Fluor 532 6
  • Alexa Fluor 568 6
  • Alexa Fluor 633 6
  • Alexa Fluor 660 6
  • Alexa Fluor 680 6
  • Alexa Fluor 750 6
  • Alexa Fluor 790 6
  • Cy5.5 6
  • Cy7 6
  • CF®640R 5
  • AF647 4
  • CF®350 4
  • CF®488A 4
  • CF®543 4
  • CF®555 4
  • CF®568 4
  • CF®594 4
  • CF®633 4
  • CF®647 4
  • CF®680 4
  • AF555 3
  • Agarose 1
  • Cy2 1
  • DyLight® 550 1
  • DyLight® 594 1
  • human 975
  • mouse 747
  • rat 421
  • rabbit 345
  • goat 278
  • cow 178
  • sheep 172
  • guinea pig 155
  • horse 132
  • cat 120
  • hamster 106
  • chicken 79
  • dog 72
  • swine 59
  • all species 20
  • pig 11
  • Secondary antibody
  • goat 2169
  • rabbit 1602
  • donkey 283
  • horse 84
  • mouse 84
  • rat 40
  • sheep 34
  • guinea pig 29
  • cow 27
  • human 27
  • hamster 26
  • cat 25
  • chicken 23
  • dog 18
  • pig 16
  • swine 11
  • IHC 2412
  • WB 2291
  • ELISA 2150
  • IF 1626
  • FC 1332
  • FLISA 668
  • Microscopy 533
  • ICC 80
  • Dot blot 42
  • ELISPOT 30
  • Assay 26
  • Stimulation 25
  • IP 8
  • Staining 6
  • ChIP 4
  • Mass spectrometry 4
  • array 4
  • Chemiluminescence 1
  • Immunodiffusion 1
  • Radioimmunoassay 1
  • Polyclonal 3442
  • polyclonal 556
  • POlyclonal 53
  • NI 33 (HP 65) 12
  • 15H6 6
  • B-Ly1 6
  • NI 330 (HP 6050) 6
  • SJ25-C1 6
  • M 2A1 4
  • M2A1 4
  • SB73a 4
  • UHB 4
  • HP6200 1
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Katalog-Nummer
Beschreibung
Cond.
Preis zzgl. MwSt.
NB-47-05566-1MG
 1mg 
NB-47-05924-500UG
 0.5mg 
NB-47-05977-500UG
 0.5mg 
NB-47-06075-800UG
 0.8mg 
NB-47-05987-500UG
 0.5mg 
NB-47-05883-800UG
 0.8mg 
NB-47-06011-500UG
 0.5mg 
NB-47-05981-500UG
 0.5mg 
NB-47-05881-400UG
 0.4mg 
NB-47-06077-600UG
 0.6mg 
NB-47-06010-500UG
 0.5mg 
NB-47-06014-500UG
 0.5mg 
NB-47-05989-500UL
 0.5ml 
NB-47-06078-1ML
 1ml 
NB-47-06031-1MG
 1mg 
NB-47-05933-500UG
 0.5mg 
NB-47-05988-500UG
 0.5mg 
NB-47-06006-1MG
 1mg