ELISPOT kits

ELISPOT kits


Enzyme-linked immunospot (ELISpot) assays are highly sensitive immunoassays that measure the frequency of individual cytokine-secreting cells. In these assays, cells are cultured on a surface coated with a specific capture antibody in the presence or absence of stimuli. Proteins, such as cytokines, that are secreted by the cells will be captured by the specific antibodies on the surface. After an appropriate incubation time, cells are removed and the secreted molecule is detected using a detection antibody in a similar procedure to that employed by the ELISA. The detection antibody is either biotinylated and followed by a streptavidin-enzyme conjugate or the antibody is directly conjugated to an enzyme. By using a substrate with a precipitating rather than a soluble product, the end result is visible spots on the surface. Each spot corresponds to an individual cytokine-secreting cell.

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RUOCE / IVD
  • Unconjugated 14769
  • Biotin 88
  • AP 35
  • HRP 32
  • Low Endotoxin/Azide Free 24
  • FITC 6
  • APC 4
  • PE 4
  • TRITC 2
  • BGAL 1
  • human 7364
  • mouse 4029
  • rat 2619
  • all species 377
  • cow 356
  • pig 294
  • dog 172
  • rabbit 156
  • chicken 111
  • monkey 63
  • horse 61
  • sheep 40
  • guinea pig 32
  • goat 16
  • cat 2
  • human 838
  • mouse 218
  • rat 152
  • goat 110
  • monkey 28
  • rabbit 15
  • cow 4
  • chicken 2
  • sheep 2
  • horse 1
  • Primary antibody 14808
  • ELISA/ELISPOT 1081
  • Secondary antibody 150
  • Discontinued 7
  • kit 4
  • Protein/peptide 2
  • Buffers and reagents 1
  • Equipment 1
  • ELISA 15817
  • ELISPOT 14966
  • Immunoassay 14546
  • IHC 230
  • WB 198
  • FC 184
  • ICC 139
  • IP 52
  • FA 24
  • Stimulation 20
  • FLISA 12
  • Neutralization 10
  • Cell depletion 8
  • Multiplex assay 8
  • Purification 7
  • Chemiluminescence 6
  • Detection 6
  • Microscopy 4
  • IF 3
  • Assay 2
  • Blocking 2
  • Proximity Ligation Assay 1
  • Quality Tested:
    ELISA
    FLISA

    Report in literature:
    ELISpot
    FC
    ICC
    WB
    Multiplex
    1
  • Polyclonal 133
  • Monoclonal 22
  • 23G3 5
  • [B-B1] 5
  • [B-B51] 5
  • [B-C49] 5
  • [B-C7] 5
  • [B-E48] 5
  • [B-E8] 5
  • [B-F60] 5
  • [B-F7] 5
  • [B-G1] 5
  • [B-G46] 5
  • [B-P24] 5
  • [B-P6] 5
  • [B-R14] 5
  • [B-T10] 5
  • 1B4B1 4
  • 2A8F4 4
  • G11C5 4
  • H2 4
  • HP6029 4
  • JES6-5H4 4
  • TRFK5 4
  • XMG1.2 4
  • 1D11.16.8 3
  • 48110 3
  • CC301 3
  • CC318 3
  • JES5-16E3 3
  • MD-1 3
  • MP4-25D2 3
  • MP6-XT3 3
  • [31-7-4] 3
  • [B-C48] 3
  • [B-G5] 3
  • 1A7 2
  • 3E6 2
  • 5D10 2
  • BVD2-21C11 2
  • BVD2-23B6 2
  • BVD4-1D11 2
  • BVD6-24G2 2
  • C3F12 2
  • CC330 2
  • DB-12 2
  • JES1-5A10 2
  • JES10-5A2 2
  • JES3-12G8 2
  • JES3-9D7 2
  • JES5-2A5 2
  • JES6-1A12 2
  • MP1-22E9 2
  • MP2-43D11 2
  • MP2-8F8 2
  • MP5-20F3 2
  • MP5-20F3 & MP5-32C11 2
  • MP5-32C11 2
  • MQ2-13A5 2
  • MQ2-39C3 2
  • QS-5 2
  • TC11-18H10 2
  • TC11-8H4 2
  • TRFK4 2
  • TRFK5 & JES1-5A10 2
  • TRFK5 & TRFK4 2
  • [5D10] 2
  • [B-B13] 2
  • [B-D48] 2
  • [B-E4] 2
  • [B-G28] 2
  • [B-S10] 2
  • [B-S23] 2
  • [B-T21] 2
  • [B-T24] 2
  • [B27] 2
  • [CC301] 2
  • [CC318] 2
  • [CC330] 2
  • [HP6002] 2
  • [HP6029] 2
  • [JES1-39D10] 2
  • [MP6-XT22] 2
  • 11B11 1
  • 11B11 & BVD6-24G2 1
  • 4F12 1
  • 5B5 1
  • A5D5 1
  • B-B13 1
  • B-F7 1
  • B-G5 1
  • B-P24 1
  • B-S10 1
  • B27 1
  • DB-1 1
  • DB-10 1
  • DB-14 1
  • DL-8 1
  • HP6061 1
  • J1B10 1
Katalog-Nummer
Beschreibung
Cond.
Preis zzgl. MwSt.
KA3937
 1Kit 
KA3936
 1Kit