Cobalt-NTA FPLC columns (Co-NTA)

Cobalt-NTA FPLC columns (Co-NTA)

Generon HiFliQ columns pre-packed and ready to use with pre-charged super Cobalt-NTA Agarose resin for affinity purification of poly-histidine tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). Available in 1 ml and 5 ml HiFliQ column sizes with high binding capacity and minimal ion leakage. Compatible with all common chromatography HPLC and FPLC instruments (including ÄKTA FPLC's), and low pressure pumps and syringes using an appropriate adaptor.

Cobalt NTA resin generally has a lower affinity for the 6x His Tag (proteins elute at a lower Imidazole concentration) but due to its slightly altered binding characteristcs can sometimes provide an increased putiy product when compared to Nickel-NTA resin.
HiFliQ Co-NTA  Ni-NTA-coordination 
Specifications: 
  • Column volume: 1 or 5 ml resin
  • Column construction: Polypropylene 
  • Resin: Super Co-NTA Agarose  
  • Base Matrix: 7.5% cross-linked Agarose 
  • Co-NTA capacity: 40-50 mg (per 1 ml resin)
  • Flow rate: 1 ml/min (1 ml), 1-5 ml/min (5 ml) 
  • Max pressure: 0.5 MPa (72 psi) 
  • Universal 10.32 (1/16'') UNF threads: Inlet Female/Outlet Male 
  • Dimensions: 15 x 80 mm (1 ml), 23 x 80 mm (5 ml)

Liste der Artikel

Katalog-Nummer
Beschreibung
Cond.
H. T. Preis
HiFliQ1-CoNTA-1
 1 column 
HiFliQ1-CoNTA-5
 5 columns 
HiFliQ5-CoNTA-1
 1 column 
HiFliQ5-CoNTA-5
 5 columns