2×TransTaq® High Fidelity (HiFi) PCR SuperMix II (-dye)

Katalog-Nummer AS131-22

Size : 5×1ml

Marke : TransGen Biotech

Contact local distributor :


Telefonnummer : +1 850 650 7790

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Product Details

TransTaq® High Fidelity (HiFi) PCR SuperMix I or II is a ready-to-use mixture of TransTaq® High Fidelity (HiFi) DNA polymerase, dNTPs and optimized buffer. TransTaq® High Fidelity (HiFi) PCR SuperMix I is optimized for the amplification of genomic DNA and PCR SuperMix II is optimized for the amplification of λDNA, cDNA or plasmid DNA. The SuperMix is  provided at 2× concentration and can be used at 1× concentration by adding template, primers and H2O.


TransTaq® High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.

• Extension rate is about 1-2 kb/min.

• Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.

• Amplification of genomic DNA fragment up to 15 kb.


Applications

• Complex templates

• GC/AT-rich templates

• Long PCR

• High yield PCR


Storage

at -20 ℃ for two years


Shipping

Dry ice (-70 ℃)

Product Contents


ComponentAS131-01/21AS131-02/22
TransTaq® HiFi PCR SuperMix (Mix I)/(Mix II)1 ml5×1 ml
Nuclease-free Water1 ml5 ml


Citations

Xu R , Qin R , Li H , et al. Generation of targeted mutant rice using a CRISPR-Cpf1 system[J]. Plant Biotechnology Journal, 2017 Jun, 15(6):713-717. 


Che L H , Zhang S Q , Li Y , et al. Genome-wide survey of nuclear protein-coding markers for beetle phylogenetics and their application in resolving both deep and shallow-level divergences[J]. Molecular Ecology Resources, 2017, 17(6):1342-1358.


Liu T , Sun L , Jiang B , et al. Lineage conversion of mouse fibroblasts to pancreatic α-cells[J]. Experimental & Molecular Medicine, 2017, 49(6):e350.


Hao J , Zhang C , Liang T , et al. rFliC prolongs allograft survival in association with the activation of recipient Tregs in a TLR5-dependent manner[J]. Cellular and Molecular Immunology, 2014, 11(2):206-214.


Feng Y J , Liu Q F , Chen M Y , et al. Parallel tagged amplicon sequencing of relatively long PCR products using the Illumina HiSeq platform and transcriptome assembly[J]. Molecular Ecology Resources, 2016, 16(1):91-102.

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