Human GPI (Glucose-6-phosphate isomerase) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	GPI
Gene ID:	2821
Uniprot ID:	G6PI_HUMAN
Specificity:	This kit recognizes Human GPI in samples. No significant cross-reactivity or interference between Human GPI and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway. ISGylated.
Function	In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis. Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility. Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons. It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion.
Protein Name	Glucose-6-Phosphate Isomerase Gpi Autocrine Motility Factor Amf Neuroleukin Nlk Phosphoglucose Isomerase Pgi Phosphohexose Isomerase Phi Sperm Antigen 36 Sa-36
Database Links	Reactome: R-HSA-5628897 Reactome: R-HSA-6798695 Reactome: R-HSA-70171 Reactome: R-HSA-70263
Cellular Localisation	Cytoplasm Secreted
Alternative CLIA Names	Glucose-6-Phosphate Isomerase CLIA kit Gpi CLIA kit Autocrine Motility Factor CLIA kit Amf CLIA kit Neuroleukin CLIA kit Nlk CLIA kit Phosphoglucose Isomerase CLIA kit Pgi CLIA kit Phosphohexose Isomerase CLIA kit Phi CLIA kit Sperm Antigen 36 CLIA kit Sa-36 CLIA kit GPI CLIA kit
Specificity	This kit recognizes Human GPI in samples. No significant cross-reactivity or interference between Human GPI and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	100-116	106
EDTA plasma(n=8)	89-103	95
Cell culture media(n=8)	97-111	104

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	49.63	100.91	367.25	50.25	92.18	370.12
Standard deviation	4.70	10.77	37.83	5.41	6.45	22.39
CV (%)	9.47	10.67	10.30	10.77	7.00	6.05

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance