EasyScript® First-Strand cDNA Synthesis SuperMix
Size : 100rxns×20µlsystem
Brand : TransGen Biotech
EasyScript® First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by EasyScript® RT/RI Enzyme Mix and 2×ES Reaction Mix.
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• The product obtained from 15 minutes reaction is used for qPCR; the, product obtained from 30 minutes reaction is used for PCR.
• Anchored Oligo(dT)18 Primer is specifically designed to bind to the first base next to mRNA Poly(A)+ on the 5' end with high specificity, ensuring high efficiency and success rate of first-strand cDNA synthesis.
• Random Primer (N9) or Gene Specific Primer (GSP) can be used to synthesize the first-strand cDNA.
• cDNA up to 8 kb.
Multiple copy gene detection
at -20 ℃ for two years
Dry ice (-70 ℃)
|EasyScript® RT/RI Enzyme Mix||50 μl||100 μl|
|2×ES Reaction Mix||500 μl||1 ml|
|Random Primer(N9) (0.1 μg/μl)||50 μl||100 μl|
|Anchored Oligo(dT)18 Primer (0.5 μg/μl)||50 μl||100 μl|
|RNase-free Water||500 μl||1 ml|
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