Specifications:
Background
Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The rat microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I. A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II. This antibody can detect both forms of LC3.
Description
This LC3 antibody is validated for multiple applications (WB, ICC, IH, IP, FCM, Immuno-EM) and has numerous citations. This is a monoclonal antibody targeting LC3 for FCM, ICC, IP, WB.
More information about LC3 and autophagy can be found in our Blog. In addition you may download our 
Autophagy Pathway Poster.
| Target: | LC3 |
|---|---|
| Product Type: | Antibody |
| Size: | 100 uL |
| Application: | FCM, ICC, IH, Immuno-EM, IP, WB |
| Research Area / Disease: | Autophagy |
| Conjugate: | Unlabeled |
| Antibody Type: | Monoclonal |
| Clone Number: | 4E12 |
| Concentration: | 2 mg/mL |
| Formulation: | 200 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
| Isotype: | IgG1 ĸ |
| Immunogen: | Recombinant human LC3 (MAP1LC3B: 1-120 a.a.) |
| Host Species: | Mouse |
| Species Reactivity: | Hamster, Human, Mouse, Rat |
| Source: | This antibody was purified from hybridoma (clone 4E12) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with C3H mouse lymphocyte immunized with the recombinant human LC3 [MAP1LC3B (1-120 aa)]. |
| Reactivity: | This antibody reacts with LC3 (MAP1LC3A, B) on Immunocytochemistry, Western blotting and Immunoprecipitation. |
| Gene ID Human: | 81631, 84557 |
| Gene ID Mouse: | 67443, 66734 |
| Gene ID Rat: | 64862, 362245 |
| Storage Temperature: | -20°C |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
Citations
.Suzuki, Kaori, et al. “Human Cathelicidin Peptide LL-37 Induces Cell Death in Autophagy-Dysfunctional Endothelial Cells.” The Journal of Immunology 208.9 (2022): 2163-2172
Kaminskyy, Vitaliy O. “A Quantitative Flow Cytometry–Based Method for Autophagy Detection Across the Cell Cycle.” Autophagy and Cancer. Humana, New York, NY, 2022. 65-74.
Jia, Wenhui, et al. “Brain‐Targeted HFn‐Cu‐REGO Nanoplatform for Site‐Specific Delivery and Manipulation of Autophagy and Cuproptosis in Glioblastoma.” Small (2022): 2205354.
Wu, Shan-Ying, et al. “Secretory autophagy promotes Rab37-mediated exocytosis of tissue inhibitor of metalloproteinase 1.” Journal of Biomedical Science 29.1 (2022): 1-18.
Bindschedler, Annina, et al. “Plasmodium berghei-Mediated NRF2 Activation in Infected Hepatocytes Enhances Parasite Survival.” Cellular Microbiology 2022 (2022).
Yuan, Weigang, Fenglei Jian, and Yueguang Rong. “Bifendate inhibits autophagy at multiple steps and attenuates oleic acid-induced lipid accumulation.” Biochemical and Biophysical Research Communications 631 (2022): 115-123.
Bindschedler, Annina, et al. “Plasmodium berghei-Mediated NRF2 Activation in Infected Hepatocytes Enhances Parasite Survival.” Cellular Microbiology 2022 (2022).
Ni, Yinhua, et al. “Spermidine Ameliorates Nonalcoholic Steatohepatitis through Thyroid Hormone-Responsive Protein Signaling and the Gut Microbiota-Mediated Metabolism of Bile Acids.” Journal of Agricultural and Food Chemistry (2022).
Chino, Haruka, et al. “Phosphorylation by casein kinase 2 enhances the interaction between ER‐phagy receptor TEX264 and ATG8 proteins.” EMBO reports (2022): e54801.
De Mazière, Ann, et al. “An optimized protocol for immuno-electron microscopy of endogenous LC3.” Autophagy (2022): 1-19.
Chino, Haruka, et al. “Phosphorylation by casein kinase 2 ensures ER-phagy receptor TEX264 binding to ATG8 proteins.” bioRxiv (2022).
Ni, Yinhua, et al. “Pharmacological activation of REV-ERBα improves nonalcoholic steatohepatitis by regulating intestinal permeability.” Metabolism 114 (2021): 154409.
Chen, Di, et al. “ORF3a of SARS-CoV-2 promotes lysosomal exocytosis-mediated viral egress.” Developmental Cell 56.23 (2021): 3250-3263.
Shiizaki, Kazuhiro, et al. “Calcium phosphate microcrystals in the renal tubular fluid accelerate chronic kidney disease progression.” The Journal of Clinical Investigation 131.16 (2021).
Tovar-y-Romo, Luis B. “Shudong Chen1†, Ruimin Tian 1, 2†, Dan Luo1, Zhifeng Xiao 1, Hui Li3 and Dingkun Lin 1, 2.” Mechanisms of Neuronal Recovery in the Central Nervous System (2021): 571258092.
Bork, Tillmann, et al. “Podocytes maintain high basal levels of autophagy independent of mtor signaling.” Autophagy 16.11 (2020): 1932-1948.
Sasabe, Eri, et al. “Metal nanoparticles-induced activation of NLRP3 inflammasome in human oral keratinocytes is a possible mechanism of oral lichenoid lesions.” Toxicology in Vitro 62 (2020): 104663.
Ma, Lingyan, et al. “Spermidine improves gut barrier integrity and gut microbiota function in diet-induced obese mice.” Gut Microbes 12.1 (2020): 1832857.
Chen, Shudong, et al. “Time-course changes and role of autophagy in primary spinal motor neurons subjected to oxygen-glucose deprivation: insights into autophagy changes in a cellular model of spinal cord ischemia.” Frontiers in Cellular Neuroscience 14 (2020): 38.
Manirujjaman, Md, et al. “Degradation of the tumor suppressor PDCD4 is impaired by the suppression of p62/SQSTM1 and autophagy.” Cells 9.1 (2020): 218.
Zemirli N et al. The primary cilium protein folliculin is part of the autophagy signaling pathway to regulate epithelial cell size in response to fluid flow. Cell Stress. 3, 100-109 (2019)
Vrahnas C et al. Author Correction: Increased autophagy in EphrinB2-deficient osteocytes is associated with elevated secondary mineralization and brittle bone. Nat Commun. 10, 5073 (2019)
Wise JP Jr et al. Autophagy Disruptions Associated With Altered Optineurin Expression in Extranigral Regions in a Rotenone Model of Parkinson’s Disease. Front Neurosci. 12, 289 (2018)
Kanda R et al. Expression of the glucagon-like peptide-1 receptor and its role in regulating autophagy in endometrial cancer. BMC Cancer 18, 657 (2018)
Mori H et al. Induction of selective autophagy in cells replicating hepatitis C virus genome. J Gen Virol. 99, 1643-1657 (2018)
Kajiume T, Kobayashi M, Human granulocytes undergo cell death via autophagy. Cell Death Discov. 4, 111 (2018)
Vrahnas C et al. Author Correction: Increased autophagy in EphrinB2-deficient osteocytes is associated with elevated secondary mineralization and brittle bone. Nat Commun. 10, 5073 (2019)
Hou B et al. SQSTM1/p62 loss reverses the inhibitory effect of sunitinib on autophagy independent of AMPK signaling. Sci Rep. 9, 1087 (2019)
Du TT et al. Anterior thalamic nucleus stimulation protects hippocampal neurons by activating autophagy in epileptic monkey. Aging (Albany NY). 12, 6324-6339 (2020)
Rahaman MS et al. Curcumin alleviates arsenic-induced toxicity in PC12 cells via modulating autophagy/apoptosis. Ecotoxicol Environ Saf. 200, 110756 (2020)
Rahaman MS et al. Effects of curcumin, D-pinitol alone or in combination in cytotoxicity induced by arsenic in PC12 cells. Food Chem Toxicol. 144, 111577 (2020)
Ye X et al. Lipopolysaccharide Induces Neuroinflammation in Microglia by Activating the MTOR Pathway and Downregulating Vps34 to Inhibit Autophagosome Formation. J Neuroinflammation. 17, 18 (2020)
Boukhalfa A et al. PI3KC2α-dependent and VPS34-independent Generation of PI3P Controls Primary Cilium-Mediated Autophagy in Response to Shear Stress. Nat Commun. 11, 294 (2020)