Goat anti-Sox9b (zebrafish aa248-259)
Cat# EB11443
Size : 100ug
Brand : Everest Biotech
Code | Name | Applications | Availability | Size | Tested species | Grade |
---|---|---|---|---|---|---|
EB11443 | Goat Anti-Sox9b (zebrafish aa248-259) Antibody | Pep-ELISA, IHC | 100µg specific antibody in 200µl | Human |
Target Protein
Principal Names: fb18d01, SRY-box containing gene 9b, wu:fb18d01, sox9bOfficial Symbol: Sox9b
Accession Number(s): NP_571719.1
Important Comments: No cross-reactivity with Sox9a
Immunogen
Peptide with sequence C-PLQDGIDFGAVD, from the internal region of the protein sequence according to NP_571719.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:64000.IHC: Paraffin embedded Human Skin. Recommended concentration: 5µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Zebrafish
- Western blot Guidelines
- Everest Western blot labeling
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- MSDS - All Goat antibodies
- Tissue Lysate Preparation
- Cell Lysate Preparation
- Blocking with the immunizing peptide
- LsBio IHC Protocol