Goat anti-Six3b (zebrafish)
Cat# EB07961
Size : 100ug
Brand : Everest Biotech
Code | Name | Applications | Availability | Size | Tested species | Grade |
---|---|---|---|---|---|---|
EB07961 | Goat Anti-Six3b (zebrafish) Antibody | Pep-ELISA | 100µg specific antibody in 200µl |
Target Protein
Principal Names: six3b, sine oculis homeobox homolog 3b, SIX3, cb347, six3.2, six6, sine oculis homeobox homolog 6Official Symbol: six3b
Accession Number(s): NP_571438.1
Important Comments: This antibody is not expected to cross-react with Six3a.
Immunogen
Peptide with sequence C-NRLQHHGLGQSSLR, from the internal region of the protein sequence according to NP_571438.1 .
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Not yet tested. At this stage we are dependent on researchers in the field for further characterization of this product. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Species Reactivity
Tested:Expected from sequence similarity: Zebrafish
- Western blot Guidelines
- Everest Western blot labeling
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- MSDS - All Goat antibodies
- Tissue Lysate Preparation
- Cell Lysate Preparation
- Blocking with the immunizing peptide
- LsBio IHC Protocol