Goat anti-Notch1a (zebrafish)
Cat# EB07958
Size : 100ug
Brand : Everest Biotech
Code | Name | Applications | Availability | Size | Tested species | Grade |
---|---|---|---|---|---|---|
EB07958 | Goat Anti-Notch1a (zebrafish) Antibody | Pep-ELISA | 100µg specific antibody in 200µl |
Target Protein
Principal Names: notch1a, notch homolog 1a, n1a, deadly seven, des, neurogenic locus notch homolog proteinOfficial Symbol: notch1a
Accession Number(s): NP_571516.1
Immunogen
Peptide with sequence C-QNMAPMQHQNISHH, from the internal region of the protein sequence according to NP_571516.1 .
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:64000.Western blot: Not yet tested - our routine western blotting protocol does not allow for the detection of proteins >250kDa (calculated size of approx. 262kDa according to NP_571516.1 ) and the antibody is an aspiring product.
Species Reactivity
Tested:Expected from sequence similarity: Zebrafish
- Western blot Guidelines
- Everest Western blot labeling
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- MSDS - All Goat antibodies
- Tissue Lysate Preparation
- Cell Lysate Preparation
- Blocking with the immunizing peptide
- LsBio IHC Protocol