Goat anti-TRPC7
Cat# EB09257
Size : 100ug
Brand : Everest Biotech
Code | Name | Applications | Availability | Size | Tested species | Grade |
---|---|---|---|---|---|---|
EB09257 | Goat Anti-TRPC7 Antibody | Pep-ELISA, WB, IP | 100µg specific antibody in 200µl | Chicken, Mouse |
Target Protein
Principal Names: TRPC7, transient receptor potential cation channel, subfamily C, member 7, TRP7, likley ortholog of mouse transient receptor potential cation channel, subfamily C, member 7, putative capacitative calcium channelOfficial Symbol: TRPC7
Accession Number(s): NP_065122.1; NP_001161049.1; NP_001161048.1; NP_001363830.1
Human GeneID(s): 57113
Non-Human GeneID(s): 26946 (mouse), 282822 (rat)
Important Comments: This antibody is expected to cross-react with isoform 1, 2 and 3 (NP_065122.1; NP_001161049.1; NP_001161048.1).
Immunogen
Peptide with sequence KLCKSKAKSCEND, from the internal region of the protein sequence according to NP_065122.1; NP_001161049.1; NP_001161048.1; NP_001363830.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: A 95kDa band was observed in Chicken Embryo Heart atrial and ventricular myocyte lysates (Sabourin et al,; PMID 21672930). This antibody has been successfully used in WB on Mouse (Murtaza et al.; PMID: 32882106).
Immunoprecipitation: This antibody has been successfully used in IP on lysates of Chicken Embryo Heart (Sabourin et al,; PMID: 21672930).
Species Reactivity
Tested: Chicken, MouseExpected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Specific References
This antibody has been successfully used in WB on Mouse:
Babar Murtaza, Aziz Hichami, Amira S Khan, Jiri Plesnik, Omar Sery, Alexander Dietrich, Lutz Birnbaumer, Naim A Khan
Implication of TRPC3 channel in gustatory perception of dietary lipids
Acta Physiol (Oxf). 2020 Sep 3;e13554. doi: 10.1111/apha.13554.
PMID: 32882106
This antibody has been successfully used in WB on Chicken:
Sabourin J, Antigny F, Robin E, Frieden M, Raddatz E.
Activation of Transient Receptor Potential Canonical 3 (TRPC3)-mediated Ca2+ Entry by A1 Adenosine Receptor in Cardiomyocytes Disturbs Atrioventricular Conduction.
J Biol Chem. 2012 Aug 3;287(32):26688-701.
PMID: 22692208
This antibody has been successfully used in WB and IP on Chicken:
Sabourin J, Robin E, Raddatz E.
A key role of TRPC channels in the regulation of electromechanical activity of the developing heart.
Cardiovasc Res. 2011 Jun 13. [Epub ahead of print]
PMID: 21672930
- Western blot Guidelines
- Everest Western blot labeling
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- MSDS - All Goat antibodies
- Tissue Lysate Preparation
- Cell Lysate Preparation
- Blocking with the immunizing peptide
- LsBio IHC Protocol