Lss (NM_031049) Rat Tagged ORF Clone
CAT#: RR203867
- TrueORF®
Lss (Myc-DDK-tagged ORF) - Rat lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) (Lss), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
Product Images
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | Lss |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | >RR203867 representing NM_031049 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGAGGGCACGTGTCTGCGGCGTCGTGGGGGACCCTATAAAACTGAGCCCGCCACCGATCTCACCC GCTGGCGGCTCCATAATGAGTTGGGTCGGCAGAGATGGACTTATTATCAAGCGGAGGAGGACCCTGGTCG AGAACAGACGGGGCTAGAGGCCCACTCTTTGGGACTGGACACAACAAGTTATTTCAAGAACTTACCTAAA GCTCAAACAGCCCATGAGGGGGCCCTGAACGGAGTAACCTTTTATGCCAAGCTGCAGGCTGAGGATGGAC ACTGGGCTGGTGATTATGGTGGTCCACTCTTCCTCTTGCCAGGTCTCCTGATTACGTGTCACATAGCACA CATCCCCCTGCCGGCTGGATACAGAGAGGAAATGGTACGGTACTTGCGCTCAGTGCAGCTTCCCAATGGC GGCTGGGGCTTGCACATTGAGGACAAGTCCACGGTGTTTGGGACTGCCCTGAGCTATGTGTCTCTCAGAA TCCTGGGTATTGGACCTGATGATCCTGACCTTGTGCGTGCTCGGAACATTCTTCACAAAAAAGGTGGTGC AGTGGCCATCCCTTCCTGGGGGAAGTTCTGGCTGGCTGTCCTGAATGTTTACAGCTGGGAAGGAATCAAT ACCCTCTTCCCTGAGATGTGGCTGCTTCCTGAATGGTTTCCTGCACATCCCTCCACTCTGTGGTGTCACT GCCGGCAGGTCTATCTGCCCATGAGCTACTGCTACGCCACTCGGCTAAGTGCCTCAGAGGACCCACTGGT TCAGAGCCTCCGCCAGGAACTCTATGTGGAGGATTATGCCAGCATCGATTGGCCAGCACAGAAGAACAAC GTGTGCCCCGATGACATGTACACGCCACACAGCTGGCTGCTGCACGTGGTATATGGACTCCTCAACCTGT ATGAACGTTTCCACAGTACCAGCCTGCGGAAGTGGGCCATCCAGTTGCTGTATGAACATGTCGCAGCTGA TGATCGGTTCACGAAATGCATCAGCATTGGCCCGATCTCAAAAACTGTCAACATGCTTATTCGTTGGTCA GTGGACGGACCATCCTCCCCTGCCTTCCAGGAGCACGTCTCGAGGATCAAAGATTATCTTTGGCTGGGCC TTGACGGCATGAAAATGCAGGGCACCAATGGATCACAGACCTGGGACACTTCATTTGCTGTCCAAGCCCT GCTGGAGGCAGGTGCACACCGAAGACCTGAGTTTTTGCCCTGCCTGCAGAAGGCTCACGAGTTCCTGCGG CTTTCCCAGGTCCCAGACAACAATCCTGACTACCAGAAGTATTATCGCCATATGCACAAGGGTGGTTTCC CCTTCAGCACACTGGACTGTGGCTGGATCGTTGCTGACTGCACGGCCGAGGCTTTGAAGGCTGTGCTGCT CCTGCAGGAGCGGTGTCCCTCAATCACCGAGCATGTCCCCCGAGAGCGACTCTACGATGCTGTGGCTGTG TTGTTGAGCATGAGGAATTCCGATGGAGGGTTTGCTACCTATGAGACTAAGCGTGGCGGGTATTTGCTGG AGCTGCTGAACCCCTCAGAGGTCTTTGGAGACATCATGATTGACTACACGTATGTAGAGTGTACCTCCGC AGTGATGCAGGCCCTGAGGCACTTCCGCGAGTACTTCCCAGACCACAGGGCTACAGAGATCAGGGAGACC CTCAATCAAGGCCTGGACTTCTGCCGAAAGAAGCAGAGAGCGGATGGCTCGTGGGAGGGCTCCTGGGGGG TTTGCTTCACCTATGGCACCTGGTTTGGCTTGGAAGCATTCGCTTGCATGGGACATATCTACCAAAATAG GACTGCTTGTGCAGAAGTAGCTCAGGCCTGCCACTTCCTCCTGTCGCGGCAGATGGCGGATGGGGGCTGG GGGGAGGACTTTGAGTCCTGTGAGCAGCGGCGGTACGTGCAGAGTGCCGGGTCCCAGGTCCATAGTACGT GCTGGGCCCTGCTGGGTTTGATGGCTGTCAGGCATCCCGACATCTCTGCTCAGGAGAGAGGAATCAGATG TCTGCTAGGGAAACAGCTCCCCAACGGAGACTGGCCTCAGGAGAACATCTCTGGGGTCTTCAACAAGTCC TGTGCCATCAGCTACACAAATTACAGAAACATCTTCCCCATCTGGGCCCTGGGCCGCTTCTCCAGCCTGT ATCCTGACAATACCCTTGCTGGCCACATTATCCTGACAATACCCTTGCTGGCCACATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR203867 representing NM_031049 Red=Cloning site Green=Tags(s) MTEGTCLRRRGGPYKTEPATDLTRWRLHNELGRQRWTYYQAEEDPGREQTGLEAHSLGLDTTSYFKNLPK AQTAHEGALNGVTFYAKLQAEDGHWAGDYGGPLFLLPGLLITCHIAHIPLPAGYREEMVRYLRSVQLPNG GWGLHIEDKSTVFGTALSYVSLRILGIGPDDPDLVRARNILHKKGGAVAIPSWGKFWLAVLNVYSWEGIN TLFPEMWLLPEWFPAHPSTLWCHCRQVYLPMSYCYATRLSASEDPLVQSLRQELYVEDYASIDWPAQKNN VCPDDMYTPHSWLLHVVYGLLNLYERFHSTSLRKWAIQLLYEHVAADDRFTKCISIGPISKTVNMLIRWS VDGPSSPAFQEHVSRIKDYLWLGLDGMKMQGTNGSQTWDTSFAVQALLEAGAHRRPEFLPCLQKAHEFLR LSQVPDNNPDYQKYYRHMHKGGFPFSTLDCGWIVADCTAEALKAVLLLQERCPSITEHVPRERLYDAVAV LLSMRNSDGGFATYETKRGGYLLELLNPSEVFGDIMIDYTYVECTSAVMQALRHFREYFPDHRATEIRET LNQGLDFCRKKQRADGSWEGSWGVCFTYGTWFGLEAFACMGHIYQNRTACAEVAQACHFLLSRQMADGGW GEDFESCEQRRYVQSAGSQVHSTCWALLGLMAVRHPDISAQERGIRCLLGKQLPNGDWPQENISGVFNKS CAISYTNYRNIFPIWALGRFSSLYPDNTLAGHIILTIPLLAT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_031049 |
| ORF Size | 2199 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 2851 bp |
| RefSeq ORF | 2202 bp |
| Locus ID | 81681 |
| UniProt ID | P48450 |
| Cytogenetics | 20p12 |
| MW | 83.3 kDa |
| Gene Summary | enzyme that catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| RN203867 | Lss (untagged ORF) - Rat lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) (Lss), (10 ug) | 739,00 EUR | |
| RR203867L3 | Lenti ORF clone of Lss (Myc-DDK-tagged ORF) - Rat lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) (Lss), (10 ug) | 1 038,00 EUR | |
| RR203867L4 | Lenti ORF clone of Lss (mGFP-tagged ORF) - Rat lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) (Lss), (10 ug) | 1 038,00 EUR |