Entpd1 (NM_022587) Rat Tagged Lenti ORF Clone

CAT#: RR204121L3

  • LentiORF®

Lenti ORF clone of Entpd1 (Myc-DDK-tagged ORF) - Rat ectonucleoside triphosphate diphosphohydrolase 1 (Entpd1), (10 ug)


Product Images

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Entpd1
Synonyms NTPDase-1
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
The ORF insert of this clone is exactly the same as(RR204121).
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NM_022587
ORF Size 1533 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_022587.2, NP_072109.2
RefSeq Size 4125 bp
RefSeq ORF 1536 bp
Locus ID 64519
Cytogenetics 1q54
Gene Summary has apyrase activity; may inactivate signaling mediated by extracellular nucleotides [RGD, Feb 2006]

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