Uba2 (NM_016682) Mouse Tagged ORF Clone
CAT#: MR209672
- TrueORF®
Uba2 (Myc-DDK-tagged) - Mouse ubiquitin-like modifier activating enzyme 2 (Uba2)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
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Product Images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | Uba2 |
| Synonyms | AA986091; Arx; Sae2; UBA1; Ubl1a2; Uble1b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | >MR209672 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACTGTCGCGGGGGTTGCCCCGGGAGCTGGCCGAGGCGGTGTCCGGCGGCCGGGTGCTGGTGGTTG GCGCGGGTGGGATCGGCTGTGAGCTGCTCAAGAACCTGGTGCTCACTGGCTTCTCCCACATCGACCTGAT TGATCTGGACACTATCGATGTCAGCAACCTCAACAGGCAGTTCTTGTTTCAAAAGAAACATGTGGGAAGA TCAAAGGCTCAGGTTGCCAAAGAAAGTGTGCTCCAGTTCCACCCCCAAGCCAACATTGAGGCTCACCATG ACAGCATCATGAACCCAGACTATAATGTGGAGTTTTTTCGACAGTTTATATTGGTCATGAATGCATTAGA TAACAGAGCTGCACGAAACCACGTGAATAGGATGTGTCTGGCCGCTGATGTGCCTCTCATTGAGAGCGGG ACTGCTGGGTATCTCGGACAGGTGACCACTATCAAGAAGGGTGTGACTGAGTGTTACGAATGTCACCCTA AGCCTACCCAGAGGACGTTCCCTGGCTGTACGATTCGGAACACACCTTCAGAACCTATCCATTGCATCGT ATGGGCCAAGTATTTGTTCAACCAGCTGTTTGGAGAGGAAGATGCTGATCAAGAAGTGTCTCCTGACAGA GCTGACCCTGAGGCTGCTTGGGAACCAACAGAGGCTGAAGCTAGAGCTCGAGCATCTAATGAAGACGGAG ACATTAAGCGTATTTCCACTAAGGAATGGGCTAAGTCAACTGGATATGATCCAGTTAAACTTTTTACCAA GCTTTTTAAAGATGATATTAGATATCTGCTGACAATGGACAAACTCTGGCGCAAGAGGAAACCTCCAGTT CCACTGGACTGGGCTGAAGTGCAGAGTCAAGGAGAAGCCAATGCCGATCAACAAAATGAGCCCCAGTTGG GTTTGAAAGACCAGCAGGTTCTGGATGTAAAGAGCTATGCGAGTCTGTTTTCAAAGAGCATCGAGACTCT GCGAGTCCATTTAGCGGAGAAGGGGGATGGAGCTGAGCTCATCTGGGATAAGGACGACCCACCTGCAATG GATTTTGTTACATCTGCTGCAAACCTCAGGATGCACATTTTCAGCATGAATATGAAGAGCAGGTTTGATA TAAAATCAATGGCAGGGAATATCATTCCTGCTATAGCTACTACTAATGCAGTGATCGCCGGCTTGATAGT GTTGGAAGGATTAAAGATTTTATCTGGAAAAATAGACCAGTGTAGAACTATTTTTTTGAATAAGCAGCCA AACCCAAGAAAGAAGCTCTTGGTGCCCTGTGCACTGGATCCTCCCAACACCAACTGTTACGTTTGTGCCA GCAAGCCAGAGGTGACTGTGCGGCTGAATGTCCACAAAGTGACTGTCCTCACCTTACAGGATAAGATAGT GAAAGAAAAGTTTGCTATGGTGGCACCAGATGTCCAAATTGAAGATGGGAAAGGCACAATCCTGATATCT TCAGAAGAGGGAGAGACAGAAGCCAATAACCCCAAGAAGTTGTCTGATTTTGGAATTAGAAACGGCAGCC GGCTTCAAGCAGACGACTTCCTCCAGGACTATACTTTGCTGATCAACATTCTTCATAGTGAAGATCTTGG AAAGGATGTTGAATTTGAAGTAGTTGGTGACAGCCCTGAAAAAGTGGGGCCAAAACAAGCTGAAGATGCT GCCAAAAGCATTGCCAACGGCAGCGATGACGGTGCCCAGCCTTCCACCTCCACAGCACAAGAGCAAGATG ATGTGCTCATAGTTGATTCAGACGAAGAAGGCCCTTCAAATAGCACCGACTGCAGCGGGGACGACAAGGC CCGCAAGCGGAAGCTGGAAGAGAATGAGGCTGCCAGTACCAAGAAGTGCCGCTTGGAGCAGATGGAAGAT CCAGATGACGTCATAGCGTTAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209672 protein sequence Red=Cloning site Green=Tags(s) MALSRGLPRELAEAVSGGRVLVVGAGGIGCELLKNLVLTGFSHIDLIDLDTIDVSNLNRQFLFQKKHVGR SKAQVAKESVLQFHPQANIEAHHDSIMNPDYNVEFFRQFILVMNALDNRAARNHVNRMCLAADVPLIESG TAGYLGQVTTIKKGVTECYECHPKPTQRTFPGCTIRNTPSEPIHCIVWAKYLFNQLFGEEDADQEVSPDR ADPEAAWEPTEAEARARASNEDGDIKRISTKEWAKSTGYDPVKLFTKLFKDDIRYLLTMDKLWRKRKPPV PLDWAEVQSQGEANADQQNEPQLGLKDQQVLDVKSYASLFSKSIETLRVHLAEKGDGAELIWDKDDPPAM DFVTSAANLRMHIFSMNMKSRFDIKSMAGNIIPAIATTNAVIAGLIVLEGLKILSGKIDQCRTIFLNKQP NPRKKLLVPCALDPPNTNCYVCASKPEVTVRLNVHKVTVLTLQDKIVKEKFAMVAPDVQIEDGKGTILIS SEEGETEANNPKKLSDFGIRNGSRLQADDFLQDYTLLINILHSEDLGKDVEFEVVGDSPEKVGPKQAEDA AKSIANGSDDGAQPSTSTAQEQDDVLIVDSDEEGPSNSTDCSGDDKARKRKLEENEAASTKKCRLEQMED PDDVIALD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_016682 |
| ORF Size | 1917 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016682.2, NP_057891.1 |
| RefSeq Size | 2524 bp |
| RefSeq ORF | 1917 bp |
| Locus ID | 50995 |
| UniProt ID | Q9Z1F9 |
| Cytogenetics | 7 B1 |
| MW | 70.6 kDa |
| Gene Summary | The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| MC202700 | Uba2 (untagged) - Mouse ubiquitin-like modifier activating enzyme 2 (Uba2), (10ug) | 595,00 EUR | |
| MG209672 | Uba2 (tGFP-tagged) - Mouse SUMO1 activating enzyme subunit 2 (Sae2) | 795,00 EUR | |
| MR209672L3 | Lenti ORF clone of Uba2 (Myc-DDK-tagged) - Mouse ubiquitin-like modifier activating enzyme 2 (Uba2) | 895,00 EUR | |
| MR209672L4 | Lenti ORF clone of Uba2 (mGFP-tagged) - Mouse ubiquitin-like modifier activating enzyme 2 (Uba2) | 895,00 EUR |