Sart1 (NM_016882) Mouse Tagged ORF Clone
CAT#: MR210757
- TrueORF®
Sart1 (Myc-DDK-tagged) - Mouse squamous cell carcinoma antigen recognized by T-cells 1 (Sart1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
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Product Images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | Sart1 |
| Synonyms | U5-110K |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | >MR210757 representing NM_016882 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTCGTCAAAGAAGCACCGTGGCGAGAAGGAGGCGGCCGGGACGACGGCTGCGGCCGGGACTGGGG GCACGACCGAGCAGCCGCCACGGCATCGCGAGCATAAGAAACACAAGCACCGGAGCAGCGGCGGCGGCAG CAGCGGCGGCGAACGACGGAAGCGGAGCCGGGAGCGTGGGAGTGAGCGCGGGAGCGGGCGGCGTGGGGCG GAAGCCGAGGCCCGCAGCGGCGCGCACGGTCGCGAACGAAGCCAAGCGGAGCCCTCGGAGCGGCGCGTGA AGCGGGAGAAACGCGATGACGGCTACGAGGCCGCTGCCAGCTCCAAAGCCAGCTCTGGCGATGCCTCATC CCTCAGCATTGAGGAGACCAATAAACTCCGAGCAAAGTTGGGGCTGAAGCCCTTGGAGGTCAATGCTGTT AAGAAAGAGGCGGGCACCAAGGAGGAGCCCGTGGCAGCCGATGTTATCAACCCCATGGCCTTGCGACAGC GGGAAGAACTGCGGGAGAAGCTGGCAGCTGCCAAGGAAAAACGCTTGCTGAACCAAAAATTGGGGAAAAT AAAGACACTGGGGGAGGATGACCCCTGGCTGGATGATACTGCAGCCTGGATAGAGAGGAGCCGGCAGCTA CAGAAAGAGAAGGACCTGGCAGAGAAGCGGGCTAAGCTACTAGAGGAGATGGACCAAGAGTTTGGTGTGA GCACTTTAGTGGAGGAGGAGTTTGAGCAGAGGCGACAGGACCTGTACAGTGCCCGGGACCTGCAAGGCCT CACTGTGGAACATGCCATCGATTCTTTTCGAGAAGGGGAGACTGTGGTCCTCACCCTCAAGGACAAAGGG GTACTGCAGGACGGGGAGGACGTGCTGGTGAATGTGAACATGGTGGACAAGGAGCGGGCGGACAAGAACG TGGAGCTTCGGAAAAAGAAGCCTGACTACCTGCCCTATGCGGAAGATGAGAGTGTGGACGATTTGGCACA GCAAAAACCTCGTTCTATCCTGGCCAAATATGATGAGGAACTGGAGGGCGAGCGACCACATTCCTTCCGT CTGGAGCAGGGTGGGATGGCCGATGGCCTGAGGGAACGAGAGTTAGAAGAAATCCGCACCAAGCTACGGC TGCAGGCTCAGTCTCTGAGCTCCGTAGGGCCCCGGCTTGCCTCTGAGTACCTCAGTCCCGAGGAGATGGT GACCTTCAAGAAGACCAAACGGAGAGTGAAGAAAATCCGAAAGAAGGAGAAGGAGGTGATAATGCGGGCA GATGACTTGCTGCCCCTGGGCGACCAGACTCAGGATGGGGACTTTGGATCCAGGCTCCGGGGCCGGGGTC GGCGCCGAGTTCCTGAGGTGGAGGAGGAGGCCCTTGAGGATGAGGAGAAGGACCCTGTGGCCCAGCCGCC ACCATCGGATGACACTCGAGTAGAGAACATGGACATCAGTGATGAAGAGGATGGGGGAGCTCTTCCGCCA GGGTCCCCAGAGGGGCTGGAGGAAGACGAGGCAGAGCTGGAGCTGCAGAAGCAGCTGGAGAAGGGGCGCC GGCTGCGGCAGCTGCAGCAACTGCAGCAGCTCCGAGACAGTGGTGAGAAGGTGCTGGAGATTGTGAAGAA ACTGGAGTCTCGCCAGCGCGGCTGGGAGGAGGAGGAGGACCCTGAGAGGAAGGGGACCATCGTGTTCAAT GCCACTTCTGAATTCTGCCGCACGCTGGGGGAGATCCCCACCTACGGGTTGGCTGGCAACCGCGAGGAGC AGGAAGAGCTCATGGACTTTGAAAGGGATGAGGAGCGCTCGGCCAACGGTGGCTCTGAATCAGATGGGGA AGAGAACATTGGCTGGAGCACCGTCAACCTGGATGAGGAGAAGCAGCATCAGGATTTCTCTGCTTCCTCT ACCACCATCCTGGATGAGGAGCCCATCGTGAACAGAGGGCTGGCTGCTGCCCTGCTCCTGTGTCAGAACA AAGGACTGCTGGAGACCACAGTACAAAAGGTGGCCCGGGTGAAGGCTCCCAATAAGTCACTGCCCTCAGC AGTGTACTGCATCGAGGATAAGATGGCCATTGATGACAAGTACAGCCGACGGGAGGAGTACAGGGGCTTC ACCCAGGACTTCAAGGAGAAAGATGGCTACAAGCCTGATGTTAAGATTGAATACGTGGATGAGACGGGCA GGAAACTGACTCCCAAGGAGGCATTCCGGCAGCTGTCCCACCGCTTCCACGGAAAGGGCTCAGGCAAGAT GAAGACTGAGCGGCGGATGAAGAAGCTGGATGAGGAGGCGCTCCTGAAGAAAATGAGCTCCAGTGACACG CCCCTGGGCACCGTGGCACTGCTCCAGGAGAAGCAGAAGGCCCAGAAGACACCGTATATCGTGCTCAGTG GCAGTGGCAAGAGCATGAATGCGAACACCATCACCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210757 representing NM_016882 Red=Cloning site Green=Tags(s) MGSSKKHRGEKEAAGTTAAAGTGGTTEQPPRHREHKKHKHRSSGGGSSGGERRKRSRERGSERGSGRRGA EAEARSGAHGRERSQAEPSERRVKREKRDDGYEAAASSKASSGDASSLSIEETNKLRAKLGLKPLEVNAV KKEAGTKEEPVAADVINPMALRQREELREKLAAAKEKRLLNQKLGKIKTLGEDDPWLDDTAAWIERSRQL QKEKDLAEKRAKLLEEMDQEFGVSTLVEEEFEQRRQDLYSARDLQGLTVEHAIDSFREGETVVLTLKDKG VLQDGEDVLVNVNMVDKERADKNVELRKKKPDYLPYAEDESVDDLAQQKPRSILAKYDEELEGERPHSFR LEQGGMADGLRERELEEIRTKLRLQAQSLSSVGPRLASEYLSPEEMVTFKKTKRRVKKIRKKEKEVIMRA DDLLPLGDQTQDGDFGSRLRGRGRRRVPEVEEEALEDEEKDPVAQPPPSDDTRVENMDISDEEDGGALPP GSPEGLEEDEAELELQKQLEKGRRLRQLQQLQQLRDSGEKVLEIVKKLESRQRGWEEEEDPERKGTIVFN ATSEFCRTLGEIPTYGLAGNREEQEELMDFERDEERSANGGSESDGEENIGWSTVNLDEEKQHQDFSASS TTILDEEPIVNRGLAAALLLCQNKGLLETTVQKVARVKAPNKSLPSAVYCIEDKMAIDDKYSRREEYRGF TQDFKEKDGYKPDVKIEYVDETGRKLTPKEAFRQLSHRFHGKGSGKMKTERRMKKLDEEALLKKMSSSDT PLGTVALLQEKQKAQKTPYIVLSGSGKSMNANTITK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms | CHROMATOGRAMS Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_016882 |
| ORF Size | 2418 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016882.3, NP_058578.3 |
| RefSeq Size | 5091 bp |
| RefSeq ORF | 2421 bp |
| Locus ID | 20227 |
| UniProt ID | Q9Z315 |
| Cytogenetics | 19 A |
| MW | 91.3 kDa |
| Gene Summary | Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA. Appears to play a role in hypoxia-induced regulation of EPO gene expression.[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| MC203040 | Sart1 (untagged) - Mouse squamous cell carcinoma antigen recognized by T-cells 1 (Sart1), (10ug) | 739,00 EUR | |
| MG210757 | Sart1 (tGFP-tagged) - Mouse squamous cell carcinoma antigen recognized by T-cells 1 (Sart1) | 938,00 EUR | |
| MR210757L3 | Lenti ORF clone of Sart1 (Myc-DDK-tagged) - Mouse squamous cell carcinoma antigen recognized by T-cells 1 (Sart1) | 1 038,00 EUR | |
| MR210757L4 | Lenti ORF clone of Sart1 (mGFP-tagged) - Mouse squamous cell carcinoma antigen recognized by T-cells 1 (Sart1) | 1 038,00 EUR |