Osbpl5 (BC079872) Mouse Tagged ORF Clone

CAT#: MR211031

  • TrueORF®

Osbpl5 (Myc-DDK-tagged) - Mouse oxysterol binding protein-like 5 (cDNA clone MGC:100260 IMAGE:6832073)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

Datasheet
SDS
Plasmid Map

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Product Images

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Osbpl5
Synonyms ORP5, Osbp2, Obph1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211031 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGAGGAGGCCTTTCTCCGGCGCCGTTTCTCGCTGTGTCCACCAGCTTCTACTCCACAGAAGACTG
ACCCCCGGAAAGTCCCACGGAACCTGCTTCTGGGCTGCGAAAATGAGCTTGGTCCCATCACCCCAGGAAG
GGACATGGAGTCCAACGGCCCATCACAACCCAGAGATGAAGAGCCCCAGACCCCAGGCTCCGCCACGAAG
GTGCCACTGGCTGAGTACAGGCTATGCAATGGATCTGACAAGGAATGTACCTCTCCAACGACCAGGGTCT
CCAAGAAGGATGCTCTCAAGGCACAGAAGGAGAACTATCGGCAGGAGAAGAAGCGGGCCACGAAGCAGCT
GTTCAGTGCCCTGACTGACCCCAGTGTGGTCATCATGGCTGACAGCCTAAAGATCCGAGGGACCCTGAAG
AGCTGGACGAAGCTGTGGTGTGTACTGAAGCCAGGGGTGCTGCTCATTTACAAGACACCCAAGGTGGGCC
AGTGGGTGGGCACGGTGCTGCTGCACTGCTGCGAGCTTATTGAGCGCCCCTCCAAGAAGGACGGCTTCTG
CTTCAAACTCTTCCACCCGCTGGACCAGTCTGTGTGGGCTGTGAAGGGCCCCAAAGGCGAGAGTGTGGGC
TCCATCACTCAGCCCCTCCCTAGCAGCTACCTGATCTTCAGGGCCGCTTCTGAATCAGACGGCCGCTGCT
GGCTAGATGCCCTGGAGCTGGCCCTTCGCTGCTCCAGCCTCCTGCGACTGAGCACGTGCAAGCAGGGTCG
AGATGGGGAGCAGGGGTCCTCGCCTGATGCCTCTCCCTCTTCTCTCTACGGACTGCCCACCTCGGCCACC
ATCCCAGATCAAGACCTGTTCCCGCTGAATGGGTCTGCCTTGGAGAACGATGCATTCTCAGACAAGTCAG
AACGAGAGAATGCAGAAGACTCGGATGCCGAGACCCAGGATCACAGCCGCAAGACCAACGAGAGTGGGAG
CGACCTGCTAGACAGCCCTGGTGGGCCATGGAGGGGAACTACCTATGTGGAACAGGTCCAGGAGGAGCTG
GGAGAGCTGGACGAGACTTCCCAGGTGGAGACAGTGTCAGAGGAAAACAAGAGCCTGATGTGGGTGCTGC
TGCGACAGCTGCGGCCAGGCATGGACCTGTCCCGTGTGGTGCTGCCGACCTTCGTGTTGGAGCCTCGGTC
CTTCCTCGGCAAGCTCTCTGATTACTACTACCACGGAGACCTGCTCTCCAGGGCAGCAGCAGAGGATGAC
CCCTACTGCCGCATGAAGCTTGTGCTCCGGTGGTACCTGTCTGGCTTCTATAAGAAGCCCAAGGGCATCA
AGAAACCCTATAATCCCATTCTGGGGGAGACTTTTCGCTGCCGCTGGCTGCACCCTCAGACCAACAGTCA
CACCTTCTACATAGCAGAACAGGTGTCTCACCATCCTCCTGTGTCTGCCTTCTATGTCAGCAACAGGAAA
GATGGCTTCTGCATGAGTGGCAGCATCACAGCAAAGTCTAAGTTCTATGGGAACTCATTGTCGGCGCTTC
TGGACGGCAAGGCTAAACTCACCTTCCTTAATCGCAAGGAGGAGTATACCCTCACCATGCCCTATGCCCA
CTGCCGAGGGATCCTCTACGGCACCATGACCATGGAGCTGGGAGGCAAAGTGAACATCGAGTGTGAGAAG
AACAACCTCCAAGCTGAACTGGACTTCAAGCTCAAGCCTTTCTTTGGAAGCAGCGCCAACATCAACCAGA
TTTCCGGGAAGATCATGTCAGGAGAGGAAGTCCTGGCACGCCTCACTGGACACTGGGACAGAGATGTATT
TATCAAGGAAGAGAGCAGTGGAGGCACCGAGCTCTTCTGGACTCCGAGTGAGGAGGTCCGCAGGCAGCGG
CTGAAGCGGCACACAGTGCTGTTGGAAGAGCAGAGTGAACTGGAGTCTGAGAGGCTGTGGCAGCATGTCA
CCAGGGCCATCCGTGAGGGTGACCAGCACAAAGCCACACAGGAGAAGTCTGTGCTGGAAGAGGCGCAGCG
GCAGCGTGCCCGTGAGCACCAGCAGAGCCTCACTCCATGGAAGCCACAGTTGTTCTTACTGGACCCTCTC
ACTCAGGAATGGCGCTACCGATATGAGGACCTCAGCCCCTGGGACCCGCTGAAGGACATTGCCCAATATG
AGCAAGATGGGATTCTTCATACTCTACAGAGGGAGACTATGTCTGGCCAGACAACCTTTCTGGGGAGTCC
TGACTCCAGGCACAAGAGGCCTAGCTCTGATCGGCGGCTCCGCAAAGCCAGTGACCAGCCCTCTGGCCAT
AGCCAGGTCACTGAGAGCAGCGGCTCCACACCTGAGTCCTGCCCAGACCTCTCTGATGAGGACTTTGTTC
CTGGTGGTGAGAGCCCATGTCCTCGGTGCAGGCGAGAGGTACACCGGCTAAAGATGCTACAAGAGGCCGT
GCTGTCCATTCAGGAAGCTCAGCAAGAGCTGCATAGGCACCTTTCGACCATGTTAAGCTCCACAGTGCGT
GCTGGGCAGGCGCCAGCCCCCAGCCTCCTGCAGAATCCCCGCTCCTGGTTCCTGCTCTGCATCTTCCTGA
CGTGTCAGCTATTCATTAACTATATCCTCAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>MR211031 protein sequence
Red=Cloning site Green=Tags(s)

MKEEAFLRRRFSLCPPASTPQKTDPRKVPRNLLLGCENELGPITPGRDMESNGPSQPRDEEPQTPGSATK
VPLAEYRLCNGSDKECTSPTTRVSKKDALKAQKENYRQEKKRATKQLFSALTDPSVVIMADSLKIRGTLK
SWTKLWCVLKPGVLLIYKTPKVGQWVGTVLLHCCELIERPSKKDGFCFKLFHPLDQSVWAVKGPKGESVG
SITQPLPSSYLIFRAASESDGRCWLDALELALRCSSLLRLSTCKQGRDGEQGSSPDASPSSLYGLPTSAT
IPDQDLFPLNGSALENDAFSDKSERENAEDSDAETQDHSRKTNESGSDLLDSPGGPWRGTTYVEQVQEEL
GELDETSQVETVSEENKSLMWVLLRQLRPGMDLSRVVLPTFVLEPRSFLGKLSDYYYHGDLLSRAAAEDD
PYCRMKLVLRWYLSGFYKKPKGIKKPYNPILGETFRCRWLHPQTNSHTFYIAEQVSHHPPVSAFYVSNRK
DGFCMSGSITAKSKFYGNSLSALLDGKAKLTFLNRKEEYTLTMPYAHCRGILYGTMTMELGGKVNIECEK
NNLQAELDFKLKPFFGSSANINQISGKIMSGEEVLARLTGHWDRDVFIKEESSGGTELFWTPSEEVRRQR
LKRHTVLLEEQSELESERLWQHVTRAIREGDQHKATQEKSVLEEAQRQRAREHQQSLTPWKPQLFLLDPL
TQEWRYRYEDLSPWDPLKDIAQYEQDGILHTLQRETMSGQTTFLGSPDSRHKRPSSDRRLRKASDQPSGH
SQVTESSGSTPESCPDLSDEDFVPGGESPCPRCRREVHRLKMLQEAVLSIQEAQQELHRHLSTMLSSTVR
AGQAPAPSLLQNPRSWFLLCIFLTCQLFINYILK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC079872
ORF Size 2622 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC079872, AAH79872
RefSeq Size 3834 bp
RefSeq ORF 2624 bp
Locus ID 79196
Cytogenetics 7 88.29 cM
MW 98.9 kDa
Gene Summary Lipid transporter involved in lipid countertransport between the endoplasmic reticulum and the plasma membrane: specifically exchanges phosphatidylserine with phosphatidylinositol 4-phosphate (PI4P), delivering phosphatidylserine to the plasma membrane in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds phosphatidylserine and PI4P in a mutually exclusive manner. May cooperate with NPC1 to mediate the exit of cholesterol from endosomes/lysosomes. Binds 25-hydroxycholesterol and cholesterol.[UniProtKB/Swiss-Prot Function]

Other Versions

SKU Description Size
MC220664 Osbpl5 (untagged) - Mouse oxysterol binding protein-like 5 (cDNA clone MGC:100260 IMAGE:6832073), (10ug)
    • 10 ug

881,00 EUR

MG211031 Osbpl5 (tGFP-tagged) - Mouse oxysterol binding protein-like 5 (cDNA clone MGC:100260 IMAGE:6832073)
    • 10 ug

1 000,00 EUR

MR211031L3 Lenti ORF clone of Osbpl5 (Myc-DDK-tagged) - Mouse oxysterol binding protein-like 5 (cDNA clone MGC:100260 IMAGE:6832073)
    • 10 ug

1 100,00 EUR

MR211031L4 Lenti ORF clone of Osbpl5 (mGFP-tagged) - Mouse oxysterol binding protein-like 5 (cDNA clone MGC:100260 IMAGE:6832073)
    • 10 ug

1 100,00 EUR