Olfr43 (NM_146711) Mouse Tagged ORF Clone

CAT#: MR213679

  • TrueORF®

Olfr43 (Myc-DDK-tagged) - Mouse olfactory receptor 43 (Olfr43)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK


Plasmid Map


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Product Images

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Olfr43
Synonyms IA7; MOR125-1; MOR125-5_p; Olfr403; RP23-20M18.3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR213679 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAGAAGAAAACGAGTCTTCTACCACAGATTTCACTCTCCTGGGAGTTACAAGGCAGCGGGAGCAGG
AATATTTCTTCTTCATCCTCTTCCTGTTCATTTACCCCATCACAGTGTTTGGGAACATGCTCATCATCCT
AGCCATTCACTCTGACACTCGCCTCCATAACCCTATGTACTTTTTCCTGGCCAACCTCTCCCTTGTTGAC
ATCTTCTTCTCCTCTGTAACTATTCCCAAGATGCTGGCCAACCATCTCCTAGGTAGCAAGGCCATCTCCT
TTGGGGGATGTATGGCACAGATGTACTTCATGATAGGCTTGGCAAATACAGACAGTTATATACTAGCTGC
AATGGCATATGACCGAGCTGTGGCTATCAGTCGCCCACTTCATTATGCAACAATTATGAGTCCACAACTT
TGTGTCCTGCTGGTTGCTGGGTCCTGGGTGATTGCAAATGCTAATGCACTGCCCCACACCCTACTCACAG
CTAGACTGTCCTTCTGTGGCAATAAGGATGTGGCCAACTTCTACTGTGACATTACACCTTTGCTCCAGCT
GTCCTGTTCTGACATCCGCTTCAATGTGAAGATGATGTACCTTGGGGTGGGGGTCTTCTCTGTGCCACTG
CTGTGCATCATCATCTCCTATGTCCGGGTCTTTTCCACAGTCTTGCGGGTTCCATCTACCAAGGGCTTCC
TGAAGGCCTTGTCCACCTGTGGCTCTCACCTGACAGTGGTGTCCTTGTATTATGGGACAGTCATGGGCAT
GTATTTCCGGCCCCTGACCAGTTACAGTCTGAAGCATGCATTGATAACTGTGATGTACACGGCAGTGACC
CCAATGCTGAACCCTTTCATCTACAGCCTGAGGAACCGGGACATGAAGGCTGCTCTGAAGAAACTCTTCC
ACTGCCCCTCTTCCTCTTCCTCCCTCATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR213679 protein sequence
Red=Cloning site Green=Tags(s)

MREENESSTTDFTLLGVTRQREQEYFFFILFLFIYPITVFGNMLIILAIHSDTRLHNPMYFFLANLSLVD
IFFSSVTIPKMLANHLLGSKAISFGGCMAQMYFMIGLANTDSYILAAMAYDRAVAISRPLHYATIMSPQL
CVLLVAGSWVIANANALPHTLLTARLSFCGNKDVANFYCDITPLLQLSCSDIRFNVKMMYLGVGVFSVPL
LCIIISYVRVFSTVLRVPSTKGFLKALSTCGSHLTVVSLYYGTVMGMYFRPLTSYSLKHALITVMYTAVT
PMLNPFIYSLRNRDMKAALKKLFHCPSSSSSLM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_146711
ORF Size 942 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_146711.1, NM_146711.2, NP_666922.2
RefSeq Size 1092 bp
RefSeq ORF 942 bp
Locus ID 258706
Cytogenetics 11 B5
MW 35.1 kDa
Gene Summary Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

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