RPUSD4 (NM_032795) Human Tagged Lenti ORF Clone
CAT#: RC204352L4
- LentiORF®
Lenti ORF clone of Human RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1, mGFP tagged
Product Images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Symbol | RPUSD4 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data | The ORF insert of this clone is exactly the same as(RC204352). |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_032795 |
| ORF Size | 1131 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032795.1 |
| RefSeq Size | 2527 bp |
| RefSeq ORF | 1134 bp |
| Locus ID | 84881 |
| UniProt ID | Q96CM3 |
| Cytogenetics | 11q24.2 |
| Domains | PseudoU_synth_2 |
| MW | 42.2 kDa |
| Gene Summary | Catalyzes uridine to pseudouridine isomerization (pseudouridylation) of different mitochondrial RNA substrates. Acts on position 1397 in 16S mitochondrial ribosomal RNA (16S mt-rRNA). This modification is required for the assembly of 16S mt-rRNA into a functional mitochondrial ribosome (PubMed:27974379). Acts on position 39 in mitochondrial tRNA(Phe) (PubMed:28082677). As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mt-rRNA, controls 16S mt-rRNA abundance and is required for intra-mitochondrial translation (PubMed:27667664).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| RC204352 | RPUSD4 (Myc-DDK-tagged)-Human RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1 | 457,00 EUR | |
| RC204352L3 | Lenti ORF clone of Human RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1, Myc-DDK-tagged | 757,00 EUR | |
| RG204352 | RPUSD4 (tGFP-tagged) - Human RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1 | 657,00 EUR | |
| SC103625 | RPUSD4 (untagged)-Human RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1 | 457,00 EUR |