2-Aminoethyl Methanethiosulfonate, Hydrochloride (MTSEA-Chloride)
Cat# A1377-70-25mg
Size : 25mg
Brand : US Biological
Grade
Highly PurifiedShipping Temp
RTStorage Temp
-20°CMethanesulfonothioic Acid S-(2-Aminoethyl) Ester Hydrochloride; MTSEA-Chloride
Reacts specifically and rapidly with thiols to form mixed disulfides. Used to probe the structures of the ACh receptor channel of the GABA receptor channel and of lactose permease. Useful for mapping the pore-lining regions of the ryanodine receptor.
Synonyms:
Methanesulfonothioic Acid S-(2-Aminoethyl) Ester Hydrochloride; MTSEA-Chloride
CAS No:
37597-96-9
Molecular Formula:
C₃H₁₀ClNO₂S₂
Molecular Weight:
191.70
Purity:
≥98%
Appearance:
Off-White to pale beige solid
Melting Point:
As Reported
Solubility:
DMSO, Methanol
Method for Determining Identity:
Proton NMR Spectroscopic and Mass Spectrometric Analysis
TLC Conditions:
As Reported
C18; Methanol: Water: Acetic acid=9:1:0.5; Visualized with Ninhydrin and KMnO4; Single spot, Rf=0.55
C18; Methanol: Water: Acetic acid=9:1:0.5; Visualized with Ninhydrin and KMnO4; Single spot, Rf=0.55
Chloride Content:
~19%
Atmosphere:
Inert Gas
Storage and Stability:
May be stored at RT for short-term only. Long-term storage is recommended at -20°C. Stable for 6 months after receipt. Store under inert atmosphere.
At pH 7.5 and room temperature, MTSEA hydrolyzes with a half-life of about 15 minutes, MTSET hydrolyzes with a half-life of 10 minutes, and MTSES hydrolyzes with a half-life of 20 minutes. Routinely, one can use 2.5mM MTSEA, 1mM MTSET, or 10mM MTSES, applied for 1 to 5 minutes. (MTSET is 2.5 times as reactive with small sulfhydryl compounds as is MTSEA, and 10 times as reactive as MTSES). (Stauffer and Karlin, 1994; Karlin, personal communication).
For application to Xenopus Oocytes, dissolve the compounds in 115mM NaCl, 2.5mM KCl, 1.8mM MgCl2, 10mM Hepes, pH 7.5 with NaOH. MTS reagents decompose in buffer very quickly.
•DMSO is a good solvent for the MTS reagents which are not water soluble (i.e. the non-charged MTS reagents).
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards:
All products should be handled by qualified personnel only, trained in laboratory procedures.
All products should be handled by qualified personnel only, trained in laboratory procedures.
Source
Synthetic
Purity
≥98%
Form
Off-White to pale beige solid
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.