HSP70 ELISA Kit (Sheep)

Cat# OKWB00440

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for HSP70 ELISA Kit (Sheep) (OKWB00440)
Product Info
Predicted Species ReactivityOvis aries|Sheep
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)85-103%95-101%87-98%89-104%
EDTA Plasma (n=5)86-101%91-101%84-98%88-96%
Heparin Plasma (n=5)83-98%86-99%85-95%87-100%
ELISA Kit PrincipleAviva Systems Biology Hsp70 ELISA Kit (Sheep) (OKWB00440) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hsp70 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hsp70 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hsp70 captured in the well.
ELISA Kit Range3.125-200ng/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)86-10496
EDTA Plasma (n=5)91-10598
Heparin Plasma (n=5)86-10492
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
Hsp70 Microplate96 Wells (12 x 8 Well strips)
Hsp70 Lyophilized Standard2 x 200 ng
100X Hsp70 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity1.875 ng/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolHSPA1A
Gene Full Nameheat shock 70kDa protein 1A
Alias Symbolsheat shock 70 kDa protein;heat shock 70 kDa protein 1A/1B;heat shock protein 70;HSP70.
NCBI Gene Id100913152
Protein NameHeat shock protein 70
Uniprot IDH2DGR2
Protein Accession #NP_001254803
Nucleotide Accession #NM_001267874