Haptoglobin ELISA Kit (Sheep)

Cat# OKIA00188

Size : 1x96wellplate

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKIA00188
Product Info
Predicted Species ReactivitySheep
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration55 Minutes
ELISA Kit PrincipleAviva Systems Biology Haptoglobin ELISA Kit (Sheep) (OKIA00188) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Haptoglobin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Haptoglobin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Haptoglobin captured in the well.
ELISA Kit Range15.625 ng/ml - 1000 ng/ml
ELISA Kit Component
ComponentAmount
Anti-Sheep Haptoglobin Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Sheep Haptoglobin Calibrator1
Additional InformationIntended Use: The Hp test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Hp in Sheep biological samples. If the ELISA is to be used outside the intended use, the user may need to optimize for said use.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity5.047 ng/ml
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Alias SymbolsHP
Protein NameHaptoglobin

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