SepFast 16/60 3-70KDa pg column

Cat# 3-70-16x600

Size : 1

Brand : Biotoolomics

Contact local distributor :

Phone : +1 850 650 7790


SepFast gel filtration media is specially designed for high resolution and high recovery fractionation of biological molecules based on their molecular weights. Gel filtration (or called size exclusion chromatography) is a proven technique, which is widely used for size-based molecular separations.

SepFast gel filtration media has a balanced design to offer high recovery and high selectivity according to individual applications. The core advantages are:

  • High resolution
  • High selectivity
  • High recovery
  • Highly scalable
  • Excellent physical and mechanical stability
  • Cost effective

The base matrix is made of agarose or a composite of polysaccharides that have been highly cross-linked. The media is very stable to most of the chemical conditions experienced in the bioprocessing industry.

The selection guide is listed as follows:

                               SepFast GF-HS
  SepFast GF-HS-M SepFast GF-HS-L
Separation range 3x103 - 7x104 6x103 - 6x105
Application Preparative grade separation for research, process development and large-scale productions
Particle Size(µm)       20-50      20-50
Format supplied       Loose resin and pre-packed columns

References Featuring Biotoolomics SepFast GF-HS-L Products:

de Cassan, S.C., Shakri, A.R., Llewellyn, D., Elias, S.C., Cho, J.S., Goodman, A.L., Jin, J., Douglas, A.D., Suwanarusk, R., Nosten, F.H. and Rénia, L., 2015. Preclinical assessment of viral vectored and protein vaccines targeting the Duffy-binding protein region II of Plasmodium vivax. Frontiers in immunology, 6, p.348

Jin, J., Tarrant, R.D., Bolam, E.J., Angell-Manning, P., Soegaard, M., Pattinson, D.J., Dulal, P., Silk, S.E., Marshall, J.M., Dabbs, R.A. and Nugent, F.L., 2018. Production, quality control, stability, and potency of cGMP-produced Plasmodium falciparum RH5. 1 protein vaccine expressed in Drosophila S2 cells. NPJ vaccines, 3(1), p.32

Characteristics of  Gel Filtration Media

Matrix Highly cross-linked agarose and dextran composites
Particle size Around 35 µm  (in the range of 20 – 50 µm)
Operational pressure 3 bar (0.3 MPa, 42 psi) for SepFast GF-HS-L
4 bar (0.4 Mpa, 56 psi) for SepFast GF-HS-M
Operating flow velocity Normally 10-50 cm/hour
pH stability 2-14 (short term) and 3-12 (long term)
4oC – 30oC
Chemical stability All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol
Avoid Oxidizing agents
Storage The media should be stored in 20% ethanol to prevent microbial growth. Store the media at a temperature of +2oC to +8oC.

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