HiFliQ pre-packed FPLC Columns

HiFliQ pre-packed FPLC Columns

Available in 1 ml and 5 ml HiFliQ column sizes with high capacities
The HiFliQ columns are pre-packed and ready to use

 with :  
- Pre-charged Nickel-NTA and Cobalt-NTA Agarose resinfor affinity purification of poly-histidine tagged recombinant proteins by IMAC (Immobilized Metal Ion Affinity Chromatography).
- Glutathione coupled Agarose resinfor rapid affinity purification of Glutathione S-transferase (GST) -tagged proteins under native conditions.
- Protein A and Protein G Agarose FF resinfor rapid antibody purification from serum, ascites and tissue culture supernatants.
- Heparinfor for rapid affinity purification of native or recombinant proteins NEW

HiFliQ Cobalt-NTA HiFliQ Nickel-NTA HiFliQ GST HiFliQ Protein A and Protein G HiFliQ Heparin
NEW

Available in 1 ml and 5 ml HiFliQ column sizes with high capacities
High binding capacity - Minimal ion leakage - High ligand density
 Compatible with all common chromatography HPLC and FPLC instruments (including ÄKTA FPLC's), and low pressure pumps and syringes using an appropriate adaptor. Also compatible with a wide range of reducing agents, detergents and other buffer additives.
    Specifications
    • Column volume: 1 or 5 ml resin
    • Column construction: Polypropylene
    • Flow rate: 1 ml/min (1 ml), 1-5 ml/min (5 ml)
    • Max pressure: 0.5 MPa (72 psi)
    • Universal 10.32 (1/16'') UNF threads: Inlet Female/Outlet Male
    • Dimensions: 15 x 80 mm (1 ml), 23 x 80 mm (5 ml)
    • Resin : Super Ni-NTA Agarose and Super Co-NTA Agarose (Base Matrix: Agarose)
    • Ni-NTA capacity: 50-75 mg (per 1 ml resin) Co-NTA capacity: 40-50 mg (per 1 ml resin) more info
          or
    • Resin : Glutathione Agarose (Base Matrix: Agarose)
    • GST capacity: 10 mg (per 1 ml resin) more info
          or
    • Resin : Protein A Agarose FF and Protein G Agarose FF (Base Matrix: Agarose)
    • Protein A capacity (hIgG): 30 mg (per 1 ml resin) Protein G capacity (hIgG): 20 mg (per 1 ml resin) more info
          or
    • Resin : Porcine Heparin Agarose HP Resin
    • Heparin capacity: 3-5 mg (per 1 ml resin)

    Designation Cat# Columns Size Size (columns)
    Co-NTA FPLC Columns HiFliQ1-CoNTA-1 1 ml 1
    HiFliQ1-CoNTA-5 1 ml 5
    HiFliQ5-CoNTA-1 5 ml 1
    HiFliQ5-CoNTA-5 5 ml 5
    Ni-NTA FPLC Columns HiFliQ1-NiNTA-1 1 ml 1
    HiFliQ1-NiNTA-5 1 ml 5
    HiFliQ5-NiNTA-1 5 ml 1
    HiFliQ5-NiNTA-5 5 ml 5
    GST FPLC Columns HiFliQ1-GST-1 1 ml 1
    HiFliQ1-GST-5 1 ml 5
    HiFliQ5-GST-1 5 ml 1
    HiFliQ5-GST-5 5 ml 5
    Protein A FPLC Columns HiFliQ1-PA-1 1 ml 1
    HiFliQ1-PA-5 1 ml 5
    HiFliQ5-PA-1 5 ml 1
    HiFliQ5-PA-5 5 ml 5
    Protein G FPLC Columns HiFliQ1-PG-1 1 ml 1
    HiFliQ1-PG-5 1 ml 5
    HiFliQ5-PG-1 5 ml 1
    HiFliQ5-PG-5 5 ml 5
    Heparin FPLC Columns - NEW HiFliQ1-HEP-1 1 ml 1
    HiFliQ1-HEP-5 1 ml 5
    HiFliQ5-HEP-1 5 ml 1
    HiFliQ5-HEP-5 5 ml 5