NeoTaqII DNA polymerase


  • The new generation of Taq-derived DNA polymerases NB-60-0005 optimized for standard PCR applications.
  • Engineered to produce high DNA yields in shorter PCR running times (15-30 s/kb extension).
  • Lacks 3´→5´ exonuclease activity and supports reliable amplification of a wide range of DNA templates up to 6 kb.
  • Suitable for PCR under minimal optimization conditions.



Storage Conditions

  • All components should be stored at -20°C in a freezer without defrost cycles to ensure maximal shelf life.
  • The enzyme remains stable at 4°C or room temperature for up to 3 days without compromising its stability.
  • The product will remain stable until the expiry date if stored as specified.


Unit Definition

  • One unit of the enzyme is defined as the amount required to catalyze the incorporation of 10 nmoles of dNTPs into acid insoluble material in 30 minutes at 72 °C under controlled assay conditions.


Enzyme Concentration

  • The enzyme concentration is 5 U/μL, dissolved in glycerol.


Magnesium Chloride Solution

  • A provided 50 mM MgCl2 solution allows users to optimize the Mg2+ concentration in PCR setups.
  • A concentration of 2.5 mM MgCl2 is generally effective with Neo Biotech Taq II DNA polymerase.
  • Thoroughly vortex the MgCl2 solution after thawing.


Primer Design

  • PCR primers should range from 15-30 bases and flank the region of interest.
  • Primers should contain 40-60% GC content and avoid sequences that may produce internal secondary structure.
  • The 3'-ends of the primers should not be complementary to avoid primer-dimer formation.
  • Avoid three consecutive G or C nucleotides near the 3'-end to prevent non-specific primer annealing.
  • Ideally, both primers should have nearly identical melting temperatures (Tm) for efficient annealing.


DNA Template

  • The recommended amount of genomic DNA template is 10 ng to 500 ng, but as little as 5 pg can be used.
  • Lower amounts may be sufficient for amplification of less complex DNA (1-20 ng).
  • When using cDNA synthesis as a template, do not exceed 10% of the final PCR reaction volume.


Quality Control Assays

  • Taq II DNA polymerase is tested for purity, with >90% purity determined by SDS polyacrylamide gel electrophoresis and Coomassie Blue staining.
  • Genomic DNA contamination is evaluated through PCR and must not be detectable.


Nuclease Assays

  • Nuclease assays are performed using pNZY28 plasmid DNA and Neo Biotech Taq II DNA polymerase, followed by visualization on a GreenSafe Premium-stained agarose gel.
  • No visible nicking or cutting of the nucleic acid should be observed. Similar tests are conducted with the reaction buffer and MgCl2 solution.


Functional Assay

  • Taq II DNA polymerase is extensively tested for performance in PCR amplification of different-sized DNA fragments from human genomic DNA.
  • The resulting PCR products should appear as single bands on a stained agarose.