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Reagents and instruments for immunology, cell biology and molecular biology.

Anti-SPIN1 antibody (80-160 Internal)

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Cat# NB-22-5748

Size : 100µg

Contact local distributor :

Phone : +1 850 650 7790

Brand : Neo Biotech

Contact local distributor :

Phone : +1 850 650 7790

Western blot analysis of the lysates from K562 cells using SPIN1 antibody.
Western blot analysis of lysates from HeLa cells, using SPIN1 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Western blot analysis of HeLa cells using Spindlin-1 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
Western blot analysis of various cells using Spindlin-1 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
Immunofluorescence analysis of Hela cell. 1, Spindlin-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Bcl-2 monoclonal antibody (6B5) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

General Info

Host:	Rabbit
Applications:	WB/IHC/IF/ELISA
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Spindlin-1 (111-160 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:20000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	SPIN1
Gene ID:	10927
Uniprot ID:	SPIN1_HUMAN
Immunogen Region:	111-160 aa
Specificity:	Spindlin-1 Polyclonal Antibody detects endogenous levels of Spindlin-1 protein.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human SPIN1 at the amino acid range 111-160

Description

Post Translational Modifications	Phosphorylated during oocyte meiotic maturation.
Function	Chromatin reader that specifically recognizes and binds histone H3 both trimethylated at 'Lys-4' and asymmetrically dimethylated at 'Arg-8' (H3K4me3 and H3R8me2a) and acts as an activator of Wnt signaling pathway downstream of PRMT2. In case of cancer, promotes cell cancer proliferation via activation of the Wnt signaling pathway. Overexpression induces metaphase arrest and chromosomal instability. Localizes to active rDNA loci and promotes the expression of rRNA genes. May play a role in cell-cycle regulation during the transition from gamete to embryo. Involved in oocyte meiotic resumption, a process that takes place before ovulation to resume meiosis of oocytes blocked in prophase I: may act by regulating maternal transcripts to control meiotic resumption.
Protein Name	Spindlin-1 Ovarian Cancer-Related Protein Spindlin1
Cellular Localisation	Nucleus Nucleolus
Alternative Antibody Names	Anti-Spindlin-1 antibody Anti-Ovarian Cancer-Related Protein antibody Anti-Spindlin1 antibody Anti-SPIN1 antibody Anti-OCR antibody Anti-SPIN antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance