General Info
| Host: | Goat |
| Applications: | Pep-ELISA/IF |
| Reactivity: | Human/Mouse/Dog/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Goat polyclonal antibody anti-RIF1 (C-Term) is suitable for use in ELISA and Immunofluorescence research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | IF-Expression of the protein seen in the nucleus of NIH3T3 and U2OS cells. 10µg/mlELISA-antibody detection limit dilution 1:128000. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Information
| Gene Symbol: | RIF1 |
| Gene ID: | 55183 |
| Uniprot ID: | RIF1_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_060621.3; NP_001171134.1 |
| Specificity: | This antibody is expected to recognize both reported isoforms (NP_060621.3; NP_001171134.1). The immunizing peptide sequence has been optimised for cross-reactiviy in mouse. Reported variants represent identical protein: NP_001171135.1, NP_001171136. |
| Immunogen Sequence: | RNLQSRWRSPSHEN |
Description
| Function | Key regulator of TP53BP1 that plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage: acts by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs. In response to DNA damage, interacts with ATM-phosphorylated TP53BP1. Interaction with TP53BP1 leads to dissociate the interaction between NUDT16L1/TIRR and TP53BP1, thereby unmasking the tandem Tudor-like domain of TP53BP1 and allowing recruitment to DNA DSBs. Once recruited to DSBs, RIF1 and TP53BP1 act by promoting NHEJ-mediated repair of DSBs. In the same time, RIF1 and TP53BP1 specifically counteract the function of BRCA1 by blocking DSBs resection via homologous recombination (HR) during G1 phase. Also required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs. Promotes NHEJ of dysfunctional telomeres. |
| Protein Name | Telomere-Associated Protein Rif1Rap1-Interacting Factor 1 Homolog |
| Database Links | Reactome: R-HSA-5693571 |
| Cellular Localisation | NucleusChromosomeTelomereCytoplasmCytoskeletonSpindleFollowing Interaction With Tp53bp1Recruited To Sites Of Dna DamageSuch As DsbsExhibits Atm- And Tp53bp1-Dependent Localization To Uncapped Or Aberrant Telomeres And To Dna Double Strand Breaks (Dsbs)Does Not Associate With Normal Telomere StructuresLocalizes To Microtubules Of The Midzone Of The Mitotic Spindle During AnaphaseAnd To Condensed Chromosomes In Telophase |
| Alternative Antibody Names | Anti-Telomere-Associated Protein Rif1 antibodyAnti-Rap1-Interacting Factor 1 Homolog antibodyAnti-RIF1 antibody |
Information sourced from Uniprot.org
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