Anti-PRPF19 antibody (140-220 Internal)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:40000 IF 1:50-200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	PRPF19
Gene ID:	27339
Uniprot ID:	PRP19_HUMAN
Immunogen Region:	171-220 aa
Specificity:	PRP19 Polyclonal Antibody detects endogenous levels of PRP19 protein.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human PRPF19 at the amino acid range 171-220

Function	Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome. Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex. Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries. The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair. Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response. May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA. As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process. In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation. May play a role in the biogenesis of lipid droplets. May play a role in neural differentiation possibly through its function as part of the spliceosome.
Protein Name	Pre-Mrna-Processing Factor 19 Nuclear Matrix Protein 200 Prp19/Pso4 Homolog Hpso4 Ring-Type E3 Ubiquitin Transferase Prp19 Senescence Evasion Factor
Database Links	Reactome: R-HSA-6781823 Reactome: R-HSA-6781827 Reactome: R-HSA-6782135 Reactome: R-HSA-6782210 Reactome: R-HSA-72163
Cellular Localisation	Nucleus Nucleoplasm Cytoplasm Cytoskeleton Spindle Lipid Droplet Nucleoplasmic In Interphase Cells Irregularly Distributed In Anaphase Cells In Prophase Cells Uniformly Distributed But Not Associated With Condensing Chromosomes Found In Extrachromosomal Regions In Metaphase Cells Mainly Localized To The Mitotic Spindle Apparatus When Chromosomes Segregate During Anaphase When Nuclei Reform During Late Telophase Uniformly Distributed In Daughter Cells And Displays No Preferred Association With Decondensing Chromatin Recruited On Damaged Dna At Sites Of Double-Strand Break
Alternative Antibody Names	Anti-Pre-Mrna-Processing Factor 19 antibody Anti-Nuclear Matrix Protein 200 antibody Anti-Prp19/Pso4 Homolog antibody Anti-Hpso4 antibody Anti-Ring-Type E3 Ubiquitin Transferase Prp19 antibody Anti-Senescence Evasion Factor antibody Anti-PRPF19 antibody Anti-NMP200 antibody Anti-PRP19 antibody Anti-SNEV antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance