Anti-Phospho-PAK4-Ser474 antibody (410-490)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:20000 IF 1:50-200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	PAK4
Gene ID:	10298
Uniprot ID:	PAK4_HUMAN
Immunogen Region:	441-490 aa
Specificity:	Phospho-PAK4 (S474) Polyclonal Antibody detects endogenous levels of PAK4 protein only when phosphorylated at S474.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human PAK4/5/6 around the phosphorylation site of Ser474 at the amino acid range 441-490

Post Translational Modifications	Autophosphorylated on serine residues when activated by CDC42/p21 (Ref.32). Phosphorylated on tyrosine residues upon stimulation of FGFR2. Methylated by SETD6. Polyubiquitinated, leading to its proteasomal degradation.
Function	Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.
Protein Name	Serine/Threonine-Protein Kinase Pak 4 P21-Activated Kinase 4 Pak-4
Database Links	Reactome: R-HSA-428540 Reactome: R-HSA-9013148 Reactome: R-HSA-9013149 Reactome: R-HSA-9013404 Reactome: R-HSA-9013406 Reactome: R-HSA-9013407 Reactome: R-HSA-9013408 Reactome: R-HSA-9013409 Reactome: R-HSA-9013420 Reactome: R-HSA-9013423 Reactome: R-HSA-9013424
Cellular Localisation	Cytoplasm Seems To Shuttle Between Cytoplasmic Compartments Depending On The Activating Effector For Example Can Be Found On The Cell Periphery After Activation Of Growth-Factor Or Integrin-Mediated Signaling Pathways
Alternative Antibody Names	Anti-Serine/Threonine-Protein Kinase Pak 4 antibody Anti-P21-Activated Kinase 4 antibody Anti-Pak-4 antibody Anti-PAK4 antibody Anti-KIAA1142 antibody

Information sourced from Uniprot.org

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