Anti-Phospho-LCK-Tyr393 antibody (330-410)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IF 1:200-1:1000 ELISA 1:10000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	LCK
Gene ID:	3932
Uniprot ID:	LCK_HUMAN
Immunogen Region:	361-410 aa
Specificity:	Phospho-Lck (Y393) Polyclonal Antibody detects endogenous levels of Lck protein only when phosphorylated at Y393.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human Lck around the phosphorylation site of Tyr393 at the amino acid range 361-410

Post Translational Modifications	Autophosphorylated on Tyr-394, increasing enzymatic activity, this site is dephosphorylated by PTN22. Phosphorylated on Tyr-505 by CSK, decreasing activity. Dephosphorylated by PTPRC/CD45. Dephosphorylation at Tyr-394 by PTPN2 negatively regulates T-cell receptor signaling. Myristoylation is required prior to palmitoylation. Palmitoylation regulates association with the plasma membrane and could be mediated by ZDHHC2.
Function	Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP. Interacts with FYB2.
Protein Name	Tyrosine-Protein Kinase Lck Leukocyte C-Terminal Src Kinase Lsk Lymphocyte Cell-Specific Protein-Tyrosine Kinase Protein Yt16 Proto-Oncogene Lck T Cell-Specific Protein-Tyrosine Kinase P56-Lck
Database Links	Reactome: R-HSA-114604 Reactome: R-HSA-1257604 Reactome: R-HSA-1433557 Reactome: R-HSA-1433559 Reactome: R-HSA-164944 Reactome: R-HSA-167590 Reactome: R-HSA-202424 Reactome: R-HSA-202427 Reactome: R-HSA-202430 Reactome: R-HSA-202433 Reactome: R-HSA-210990 Reactome: R-HSA-2219530 Reactome: R-HSA-2424491 Reactome: R-HSA-389356 Reactome: R-HSA-389357 Reactome: R-HSA-389359 Reactome: R-HSA-389513 Reactome: R-HSA-389948 Reactome: R-HSA-6811558 Reactome: R-HSA-9013407 Reactome: R-HSA-9020558 Reactome: R-HSA-9670439 Reactome: R-HSA-9706374
Cellular Localisation	Cell Membrane Lipid-Anchor Cytoplasmic Side Cytoplasm Cytosol Present In Lipid Rafts In An Inactive Form
Alternative Antibody Names	Anti-Tyrosine-Protein Kinase Lck antibody Anti-Leukocyte C-Terminal Src Kinase antibody Anti-Lsk antibody Anti-Lymphocyte Cell-Specific Protein-Tyrosine Kinase antibody Anti-Protein Yt16 antibody Anti-Proto-Oncogene Lck antibody Anti-T Cell-Specific Protein-Tyrosine Kinase antibody Anti-P56-Lck antibody Anti-LCK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance